- THIS ARTICLE
- Full Text
- Full Text (PDF)
- Data Supplement
-
All Versions of this Article:
genetics.106.067918v1
175/4/1665 most recent - Alert me when this article is cited
- Alert me if a correction is posted
- SERVICES
- Similar articles in this journal
- Similar articles in PubMed
- Alert me to new issues of the journal
- Download to citation manager
- Reprints & Permissions
- CITING ARTICLES
- Citing Articles via HighWire
- Citing Articles via Google Scholar
- GOOGLE SCHOLAR
- Articles by Tarailo, M.
- Articles by Rose, A. M.
- Search for Related Content
- PUBMED
- PubMed Citation
- Articles by Tarailo, M.
- Articles by Rose, A. M.
Originally published as Genetics Published Articles Ahead of Print on January 21, 2007.
Genetics, Vol. 175, 1665-1679, April 2007, Copyright © 2007
doi:10.1534/genetics.106.067918
Suppressors of Spindle Checkpoint Defect (such) Mutants Identify New mdf-1/MAD1 Interactors in Caenorhabditis elegans
Maja Tarailo*,
Risa Kitagawa
and
Ann M. Rose*,1
* Department of Medical Genetics, Faculty of Medicine, University of British Columbia, Vancouver, British Columbia V6T 1Z3, Canada and Department of Molecular Pharmacology, St. Jude Children's Research Hospital, Memphis, Tennessee 38105
1 Corresponding author: Department of Medical Genetics, Faculty of Medicine, University of British Columbia Life Sciences Centre, Rm. 1364, 2350 Health Sciences Mall, Vancouver, BC V6T 1Z3, Canada.
E-mail: arose{at}gene.nce.ubc.ca
The spindle assembly checkpoint (SAC) governs the timing of metaphase-to-anaphase transition and is essential for genome stability. The Caenorhabditis elegans mutant strain gk2 carries a deletion within the mdf-1/MAD1 gene that results in death of the homozygous strain after two or three generations. Here we describe 11 suppressors of the mdf-1(gk2) lethality, 10 identified in an ethyl methanesulfonate (EMS) mutagenesis screen and 1 isolated using the dog-1(gk10) (deletions of guanine-rich DNA) mutator strain. Using time-lapse imaging of early embryonic cells and germline mitotic division, we demonstrate that there are two classes of suppressors. Eight suppressors compensate for the loss of the checkpoint by delaying mitotic progression, which coincides with securin (IFY-1/Pds1) accumulation; three suppressors have normal IFY-1/Pds1 levels and normal anaphase onset. Furthermore, in the class of suppressors with delayed mitotic progression, we have identified four alleles of known suppressors emb-30/APC4 and fzy-1/CDC20, which are components of the anaphase-promoting complex/cyclosome (APC/C). In addition, we have identified another APC/C component capable of bypassing the checkpoint requirement that has not previously been described in C. elegans. The such-1/APC5-like mutation, h1960, significantly delays anaphase onset both in germline and in early embryonic cells.
This article has been cited by other articles:
 |
|
 |
|
  M. Tarailo, S. Tarailo, and A. M. Rose Synthetic Lethal Interactions Identify Phenotypic "Interologs" of the Spindle Assembly Checkpoint Components Genetics, December 1, 2007; 177(4): 2525 - 2530. [Abstract] [Full Text] [PDF]
|
|