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Originally published as Genetics Published Articles Ahead of Print on February 4, 2007.
Genetics, Vol. 175, 1571-1584, April 2007, Copyright © 2007
doi:10.1534/genetics.106.061309
Novel mad2 Alleles Isolated in a Schizosaccharomyces pombe 
-Tubulin Mutant Are Defective in Metaphase Arrest Activity, but Remain Functional for Chromosome Stability in Unperturbed Mitosis
Yoshie Tange and Osami Niwa1
Kazusa DNA Research Institute, Kisarazu, Chiba 292-0818, Japan
1 Corresponding author: Kazusa DNA Research Institute, 2-6-7 Kazusa-kamatari, Kisarazu, Chiba 292-0818, Japan.
E-mail: niwa{at}kazusa.or.jp
A previously isolated fission yeast 
-tubulin mutant containing apparently stabilized microtubules proliferated at an approximately identical rate as wild type, yet the mutant mitosis spindle dynamics were aberrant, particularly the kinetochore microtubule dynamics. Progression through mitosis in the mutant, however, resulted in mostly accurate chromosome segregation. In the absence of the spindle assembly checkpoint gene, mad2+, the spindle dynamics in the -tubulin mutant were greatly compromised, leading to a high incidence of chromosome missegregation. Unlike in wild-type cells, green fluorescent protein (GFP)-tagged Mad2 protein often accumulated near one of the poles of an elongating spindle in the -tubulin mutant. We isolated novel mad2 mutants that were defective in arresting mitotic progression upon gross perturbation of the spindle formation but remained functional for the viability of the -tubulin mutant. Further, the mad2 mutations did not appreciably destabilize minichromosomes in unperturbed mitoses. When overexpressed ectopically, these mutant Mad2 proteins sequestered wild-type Mad2, preventing its function in mitotic checkpoint arrest, but not in minichromosome stability. These results indicated that the Mad2 functions required for checkpoint arrest and chromosome stability in unperturbed mitosis are genetically discernible. Immunoprecipitation studies demonstrated that GFP-fused mutant Mad2 proteins formed a Mad1-containing complex with altered stability compared to that formed with wild-type Mad2, providing clues to the novel mad2 mutant phenotype.
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Genetics 2007 175: NP.
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