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Originally published as Genetics Published Articles Ahead of Print on December 28, 2006.
Genetics, Vol. 175, 1335-1340, March 2007, Copyright © 2007
doi:10.1534/genetics.106.068247
Evolution of Different Y Chromosomes in Two Medaka Species, Oryzias dancena and O. latipes
Yusuke Takehana*,1,2,
Diana Demiyah*,1,
Kiyoshi Naruse
,
Satoshi Hamaguchi* and
Mitsuru Sakaizumi*
* Graduate School of Science and Technology, Niigata University, Ikarashi, Niigata 950-2181, Japan and Department of Biological Science, School of Science, University of Tokyo, Bunkyo-ku, Tokyo, 113-0033, Japan
2 Corresponding author: Department of Environmental Science, Faculty of Science, Niigata University, 8050 Ikarashi-2, Niigata, 950-2181, Japan.
E-mail: yusuke{at}env.sc.niigata-u.ac.jp
Although the sex-determining gene DMY has been identified on the Y chromosome in the medaka (Oryzias latipes), this gene is absent in most Oryzias species, suggesting that closely related species have different sex-determining genes. Here, we investigated the sex-determination mechanism in O. dancena, which does not possess the DMY gene. Since heteromorphic sex chromosomes have not been reported in this species, a progeny test of sex-reversed individuals produced by hormone treatment was performed. Sex-reversed males yielded all-female progeny, indicating that O. dancena has an XX/XY sex-determination system. To uncover the cryptic sex chromosomes, sex-linked DNA markers were screened using expressed sequence tags (ESTs) established in O. latipes. Linkage analysis of isolated sex-linked ESTs showed a conserved synteny between the sex chromosomes in O. dancena and an autosome in O. latipes. Fluorescence in situ hybridization (FISH) analysis of these markers confirmed that sex chromosomes of these species are not homologous. These findings strongly suggest an independent origin of sex chromosomes in O. dancena and O. latipes. Further analysis of the sex-determining region in O. dancena should provide crucial insights into the evolution of sex-determination mechanisms in vertebrates.
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Genetics 2007 175: NP.
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