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Originally published as Genetics Published Articles Ahead of Print on November 16, 2006.
Genetics, Vol. 175, 453-457, January 2007, Copyright © 2007
doi:10.1534/genetics.106.065201
Mutations Leading to Loss of Sporulation Ability in Bacillus subtilis Are Sufficiently Frequent to Favor Genetic Canalization
Joanna Masel1 and Heather Maughan2
Department of Ecology and Evolutionary Biology, University of Arizona, Tucson, Arizona 85721
1 Corresponding author: Department of Ecology and Evolutionary Biology, University of Arizona, 1041 E. Lowell St., Tucson, AZ 85721.
E-mail: masel{at}u.arizona.edu
We measured the rate of mutations impairing sporulation ability in Bacillus subtilis as 0.003 in a mutator population, following 6000 generations of strong selection for sporulation that have previously been described. This means that the product of the population size and the functional mutation rate is 
105, well within the parameter range for which genetic canalization of sporulation ability is expected.
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