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Originally published as Genetics Published Articles Ahead of Print on December 18, 2006.

Genetics, Vol. 175, 441-452, January 2007, Copyright © 2007
doi:10.1534/genetics.106.064360

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Transposition of a Fungal Miniature Inverted-Repeat Transposable Element Through the Action of a Tc1-Like Transposase

Marie Dufresne*,1, Aurélie Hua-Van{dagger},1, Hala Abd el Wahab*, Sarrah Ben M'Barek{ddagger}, Christelle Vasnier*, Laure Teysset§, Gert H. J. Kema{ddagger} and Marie-Josée Daboussi*,2

* Institut de Génétique et Microbiologie, Université Paris-Sud, UMR8621, F-91405 Orsay, France, {dagger} Laboratoire Evolution, Génomes et Spéciation, Centre National de la Recherche Scientifique, FR-91198 Gif-sur-Yvette, France, {ddagger} Wageningen University and Research Center, Plant Research International B.V., 6700 AA Wageningen, The Netherlands and § Institut Jacques Monod, Universités Paris 6 et 7, UMR7592, 75251 Paris, France

2 Corresponding author: Institut de Génétique et Microbiologie, Bât 400, Université Paris-Sud, F-91405 Orsay Cedex, France.
E-mail: marie-jose.daboussi{at}igmors.u-psud.fr

The mimp1 element previously identified in the ascomycete fungus Fusarium oxysporum has hallmarks of miniature inverted-repeat transposable elements (MITEs): short size, terminal inverted repeats (TIRs), structural homogeneity, and a stable secondary structure. Since mimp1 has no coding capacity, its mobilization requires a transposase-encoding element. On the basis of the similarity of TIRs and target-site preference with the autonomous Tc1-like element impala, together with a correlated distribution of both elements among the Fusarium genus, we investigated the ability of mimp1 to jump upon expression of the impala transposase provided in trans. Under these conditions, we present evidence that mimp1 transposes by a cut-and-paste mechanism into TA dinucleotides, which are duplicated upon insertion. Our results also show that mimp1 reinserts very frequently in genic regions for at least one-third of the cases. We also show that the mimp1/impala double-component system is fully functional in the heterologous species F. graminearum, allowing the development of a highly efficient tool for gene tagging in filamentous fungi.




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