Originally published as Genetics Published Articles Ahead of Print on October 22, 2006.

Genetics, Vol. 175, 335-347, January 2007, Copyright © 2007
doi:10.1534/genetics.106.064311

Quantitative Trait Loci x Maternal Cytoplasmic Environment Interaction for Development Rate in Oncorhynchus mykiss

Krista M. Nichols*,1, Karl W. Broman{dagger}, Kyle Sundin*, Jennifer M. Young*, Paul A. Wheeler* and Gary H. Thorgaard*,{ddagger}

* School of Biological Sciences and Center for Reproductive Biology, Washington State University, Pullman, Washington 99164-4236, {dagger} Department of Biostatistics, Johns Hopkins University, Baltimore, Maryland 21205-2179 and {ddagger} School of Biological Sciences and Center for Reproductive Biology, Washington State University, Pullman, Washington 99164-4236

1 Corresponding author: Department of Biological Sciences, Purdue University, 915 W. State St., West Lafayette, IN 47907. 
E-mail: kmnichol{at}purdue.edu

Effects of maternal cytoplasmic environment (MCE) on development rate in rainbow trout were evaluated within a quantitative trait loci (QTL) analysis framework. Previous research had identified QTL for development rate in doubled haploid (DH) progeny produced from a cross between the Oregon State University (OSU) and the Swanson (SW) River rainbow trout clonal lines. In this study, progeny for QTL mapping were produced from a cross between the OSU and Clearwater (CW) River clonal lines. Doubled haploids were produced from the OSU x CW F1 by androgenesis using eggs from different females (or MCEs); with androgenesis, the maternal nuclear genome was destroyed by irradiation and diploidy was restored by blocking the first embryonic cleavage by heat shock. All embryos were incubated at the same temperature and development rate quantified as time to hatch. Using a linkage map constructed primarily with AFLP markers, QTL mapping was performed, including MCE covariates and QTL x MCE effects in models for testing. The major QTL for development rate in the OSUxSW cross overlaps with the major QTL found in this OSU x CW cross; effects at this locus were the same across MCEs. Both MCE and QTL x MCE effects contribute to variability in development rate, but QTL x MCE were minor and detected only at small-effect QTL.




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