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Originally published as Genetics Published Articles Ahead of Print on October 22, 2006.
Genetics, Vol. 175, 31-39, January 2007, Copyright © 2007
doi:10.1534/genetics.106.064238
Cytological Visualization of DNA Transposons and Their Transposition Pattern in Somatic Cells of Maize
Weichang Yu1, Jonathan C. Lamb1, Fangpu Han1 and James A. Birchler2
Division of Biological Sciences, University of Missouri, Columbia, Missouri 65211
2 Corresponding author: 117 Tucker Hall, University of Missouri, Columbia, MO 65211.
E-mail: birchlerj{at}missouri.edu
Global genomic analysis of transposable element distributions of both natural (En/Spm, Ac–Ds, and MuDR/Mu) and modified (RescueMu) types was performed by fluorescence in situ hybridization (FISH) on somatic chromosomes coupled with karyotyping of each chromosome. In lines without an active transposable element, the locations of silent En/Spm, Ac–Ds, and MuDR/Mu elements were visualized, revealing variation in copy number and position among lines but no apparent locational bias. The ability to detect single elements was validated by using previously mapped active Ac elements. Somatic transpositions were documented in plants containing an engineered Mutator element, RescueMu, via use of the karyotyping system. By analyzing the RescueMu lines, we found that transposition of RescueMu in root-tip cells follows the cut-and-paste type of transposition. This work demonstrates the utility of FISH and karyotyping in the study of transposon activity and its consequences.
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Genetics 2007 175: NP.
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