- THIS ARTICLE
- Full Text
- Full Text (PDF)
-
All Versions of this Article:
genetics.106.065185v1
175/1/21 most recent - Alert me when this article is cited
- Alert me if a correction is posted
- SERVICES
- Email this article to a friend
- Related articles in Genetics
- Similar articles in this journal
- Similar articles in PubMed
- Alert me to new issues of the journal
- Download to citation manager
- Reprints & Permissions
- CITING ARTICLES
- Citing Articles via HighWire
- Citing Articles via Google Scholar
- GOOGLE SCHOLAR
- Articles by Pacher, M.
- Articles by Puchta, H.
- Search for Related Content
- PUBMED
- PubMed Citation
- Articles by Pacher, M.
- Articles by Puchta, H.
Originally published as Genetics Published Articles Ahead of Print on October 22, 2006.
Genetics, Vol. 175, 21-29, January 2007, Copyright © 2007
doi:10.1534/genetics.106.065185
Two Unlinked Double-Strand Breaks Can Induce Reciprocal Exchanges in Plant Genomes via Homologous Recombination and Nonhomologous End Joining
Michael Pacher, Waltraud Schmidt-Puchta and Holger Puchta1
Botany II, University of Karlsruhe, D-76128 Karlsruhe, Germany
1 Corresponding author: Botanisches Institut–Lehrstuhl II Fritz-Haber-Weg 4, Universität Karlsruhe, D-76128 Karlsruhe, Germany.
E-mail: holger.puchta{at}bio.uka.de
Using the rare-cutting endonuclease I-SceI we were able to demonstrate before that the repair of a single double-strand break (DSB) in a plant genome can be mutagenic due to insertions and deletions. However, during replication or due to irradiation several breaks might be induced simultaneously. To analyze the mutagenic potential of such a situation we established an experimental system in tobacco harboring two unlinked transgenes, each carrying an I-SceI site. After transient expression of I-SceI a kanamycin-resistance marker could be restored by joining two previously unlinked broken ends, either by homologous recombination (HR) or by nonhomologous end joining (NHEJ). Indeed, we were able to recover HR and NHEJ events with similar frequencies. Despite the fact that no selection was applied for joining the two other ends, the respective linkage could be detected in most cases tested, demonstrating that the respective exchanges were reciprocal. The frequencies obtained indicate that DSB-induced translocation is up to two orders of magnitude more frequent in somatic cells than ectopic gene conversion. Thus, DSB-induced reciprocal exchanges might play a significant role in plant genome evolution. The technique applied in this study may also be useful for the controlled exchange of unlinked sequences in plant genomes.
Related articles in Genetics:
ISSUE HIGHLIGHTS
Genetics 2007 175: NP.
This article has been cited by other articles:
 |
|
 |
|
  P. Zhang, W. Li, B. Friebe, and B. S. Gill The Origin of a "Zebra" Chromosome in Wheat Suggests Nonhomologous Recombination as a Novel Mechanism for New Chromosome Evolution and Step Changes in Chromosome Number Genetics, July 1, 2008; 179(3): 1169 - 1177. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. I. Boubriak, D. M. Grodzinsky, V. P. Polischuk, V. D. Naumenko, N. P. Gushcha, A. N. Micheev, S. J. McCready, and D. J. Osborne Adaptation and Impairment of DNA Repair Function in Pollen of Betula verrucosa and Seeds of Oenothera biennis from Differently Radionuclide-contaminated Sites of Chernobyl Ann. Bot., January 1, 2008; 101(2): 267 - 276. [Abstract] [Full Text] [PDF]
|
|
|