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Originally published as Genetics Published Articles Ahead of Print on October 22, 2006.
Genetics, Vol. 174, 2255-2257, December 2006, Copyright © 2006
doi:10.1534/genetics.106.063461
A New Family of Drosophila Balancer Chromosomes With a w– dfd-GMR Yellow Fluorescent Protein Marker
Tien Le*,
Zhiguo Liang
,
Heeren Patel*,
Marcus H. Yu*,
Gitanjali Sivasubramaniam*,
Matthew Slovitt*,
Guy Tanentzapf
,1,
Nihar Mohanty*,
Sarah M. Paul*,
Victoria M. Wu* and
Greg J. Beitel*,2
* Department of Biochemistry, Molecular Biology and Cell Biology, Northwestern University, Evanston, Illinois 60208, Department of Microbiology and Immunology, University of Illinois, Chicago, Illinois 60612-7344 and The Wellcome Trust/Cancer Research UK Gurdon Institute, Cambridge CB2 1QR, United Kingdom
2 Corresponding author: BMBCB, Hogan Hall, Room 2-100, Northwestern University, 2205 Tech Dr., Evanston, IL 60208-3500.
E-mail: beitel{at}northwestern.edu
We report new w– fluorescent balancers scorable from stage 13 through adulthood that bear a nuclear-localized yellow fluorescent protein marker directly driven by dfd and GMR enhancer elements. The utility of this marker is enhanced by identification of an anti-GFP/yellow fluorescent protein (YFP) serum that is compatible with heat fixation.
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