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Originally published as Genetics Published Articles Ahead of Print on April 2, 2006.
Genetics, Vol. 174, 693-705, October 2006, Copyright © 2006
doi:10.1534/genetics.105.055152
Differential Roles of Transcriptional Mediator Complex Subunits Crsp34/Med27, Crsp150/Med14 and Trap100/Med24 During Zebrafish Retinal Development
Katrin Dürr*,
Jochen Holzschuh*,
Alida Filippi*,
Anne-Kathrin Ettl*,
Soojin Ryu*,
Iain T. Shepherd
and
Wolfgang Driever*,1
* Department of Developmental Biology, Institute for Biology 1, University of Freiburg, 79104 Freiburg, Germany and Department of Biology, Emory University, Rollins Research Center, Atlanta, Georgia 30322
1 Corresponding author: Department of Developmental Biology, Institute for Biology 1, University of Freiburg, Hauptstrasse 1, D-79104 Freiburg, Germany.
E-mail: driever{at}biologie.uni-freiburg.de
The transcriptional mediator complex has emerged as an important component of transcriptional regulation, yet it is largely unknown whether its subunits have differential functions in development. We demonstrate that the zebrafish mutation m885 disrupts a subunit of the mediator complex, Crsp34/Med27. To explore the role of the mediator in the control of retinal differentiation, we employed two additional mutations disrupting the mediator subunits Trap100/Med24 and Crsp150/Med14. Our analysis shows that loss of Crsp34/Med27 decreases amacrine cell number, but increases the number of rod photoreceptor cells. In contrast, loss of Trap100/Med24 decreases rod photoreceptor cells. Loss of Crsp150/Med14, on the other hand, only slightly reduces dopaminergic amacrine cells, which are absent from both crsp34m885 and trap100lessen mutant embryos. Our data provide evidence for differential requirements for Crsp34/Med27 in developmental processes. In addition, our data point to divergent functions of the mediator subunits Crsp34/Med27, Trap100/Med24, and Crsp150/Med14 and, thus, suggest that subunit composition of the mediator contributes to the control of differentiation in the vertebrate CNS.