help button home button Genetics AJP: Cell Physiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Originally published as Genetics Published Articles Ahead of Print on September 1, 2006.

Genetics, Vol. 174, 1041-1056, October 2006, Copyright © 2006
doi:10.1534/genetics.106.060996

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Data Supplement
Right arrow All Versions of this Article:
genetics.106.060996v1
174/2/1041    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Sarkar, S. F.
Right arrow Articles by Guttman, D. S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Sarkar, S. F.
Right arrow Articles by Guttman, D. S.

Comparative Genomics of Host-Specific Virulence in Pseudomonas syringae

Sara F. Sarkar*,1, Jeffrey S. Gordon{dagger}, Gregory B. Martin{dagger} and David S. Guttman*,{ddagger},2

* Department of Botany, University of Toronto, Toronto, Ontario M5S3B2, Canada, {ddagger} Centre for the Analysis of Genome Evolution and Function, University of Toronto, Toronto, Ontario M5S3B2, Canada and {dagger} Boyce Thompson Institute for Plant Research and Department of Plant Pathology, Cornell University, Ithaca, New York 14853

2 Corresponding author: Department of Botany, University of Toronto, 25 Willcocks St., Toronto, ON M5S3B2, Canada.
E-mail: david.guttman{at}utoronto.ca

While much study has gone into characterizing virulence factors that play a general role in disease, less work has been directed at identifying pathogen factors that act in a host-specific manner. Understanding these factors will help reveal the variety of mechanisms used by pathogens to suppress or avoid host defenses. We identified candidate Pseudomonas syringae host-specific virulence genes by searching for genes whose distribution among natural P. syringae isolates was statistically associated with hosts of isolation. We analyzed 91 strains isolated from 39 plant hosts by DNA microarray-based comparative genomic hybridization against an array containing 353 virulence-associated (VA) genes, including 53 type III secretion system effectors (T3SEs). We identified individual genes and gene profiles that were significantly associated with strains isolated from cauliflower, Chinese cabbage, soybean, rice, and tomato. We also identified specific horizontal gene acquisition events associated with host shifts by mapping the array data onto the core genome phylogeny of the species. This study provides the largest suite of candidate host-specificity factors from any pathogen, suggests that there are multiple ways in which P. syringae isolates can adapt to the same host, and provides insight into the evolutionary mechanisms underlying host adaptation.




This article has been cited by other articles:


Home page
 Appl. Environ. Microbiol.Home page
S. Yan, H. Liu, T. J. Mohr, J. Jenrette, R. Chiodini, M. Zaccardelli, J. C. Setubal, and B. A. Vinatzer
Role of Recombination in the Evolution of the Model Plant Pathogen Pseudomonas syringae pv. tomato DC3000, a Very Atypical Tomato Strain
Appl. Envir. Microbiol., May 15, 2008; 74(10): 3171 - 3181.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
T. J. Mohr, H. Liu, S. Yan, C. E. Morris, J. A. Castillo, J. Jelenska, and B. A. Vinatzer
Naturally Occurring Nonpathogenic Isolates of the Plant Pathogen Pseudomonas syringae Lack a Type III Secretion System and Effector Gene Orthologues
J. Bacteriol., April 15, 2008; 190(8): 2858 - 2870.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
J. D. Lewis, W. Abada, W. Ma, D. S. Guttman, and D. Desveaux
The HopZ Family of Pseudomonas syringae Type III Effectors Require Myristoylation for Virulence and Avirulence Functions in Arabidopsis thaliana
J. Bacteriol., April 15, 2008; 190(8): 2880 - 2891.
[Abstract] [Full Text] [PDF]

Home page
 Int. J. Syst. Evol. Microbiol.Home page
N. Parkinson, V. Aritua, J. Heeney, C. Cowie, J. Bew, and D. Stead
Phylogenetic analysis of Xanthomonas species by comparison of partial gyrase B gene sequences
Int J Syst Evol Microbiol, December 1, 2007; 57(12): 2881 - 2887.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
L. E. Rose, R. W. Michelmore, and C. H. Langley
Natural Variation in the Pto Disease Resistance Gene Within Species of Wild Tomato (Lycopersicon). II. Population Genetics of Pto
Genetics, March 1, 2007; 175(3): 1307 - 1319.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2006 by the Genetics Society of America.