help button home button Genetics J Bacteriology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Genetics, Vol. 174, 29-39, September 2006, Copyright © 2006
doi:10.1534/genetics.106.060152

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Data Supplement
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kim, J. S.
Right arrow Articles by Park, B.-S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kim, J. S.
Right arrow Articles by Park, B.-S.

A Sequence-Tagged Linkage Map of Brassica rapa

Jung Sun Kim*, Tae Young Chung{dagger}, Graham J. King{ddagger}, Mina Jin*, Tae-Jin Yang*, Yong-Moon Jin*, Ho-Il Kim* and Beom-Seok Park*,1

* National Institute of Agricultural Biotechnology (NIAB), Rural Development Administration, Suwon, 441-707, Korea, {dagger} Department of Genetic Engineering, SungKyunKwan University, Suwon, 440-746, Korea and {ddagger} Rothamsted Research, Harpenden, AL5 2QJ, United Kingdom

1 Corresponding author: National Institute of Agricultural Biotechnology (NIAB), Rural Development Administration, Suwon, 441-707, Korea.
E-mail: pbeom{at}rda.go.kr

A detailed genetic linkage map of Brassica rapa has been constructed containing 545 sequence-tagged loci covering 1287 cM, with an average mapping interval of 2.4 cM. The loci were identified using a combination of 520 RFLP and 25 PCR-based markers. RFLP probes were derived from 359 B. rapa EST clones and amplification products of 11 B. rapa and 26 Arabidopsis. Including 21 SSR markers provided anchors to previously published linkage maps for B. rapa and B. napus and is followed as the referenced mapping of R1–R10. The sequence-tagged markers allowed interpretation of the pattern of chromosome duplications within the B. rapa genome and comparison with Arabidopsis. A total of 62 EST markers showing a single RFLP band were mapped through 10 linkage groups, indicating that these can be valuable anchoring markers for chromosome-based genome sequencing of B. rapa. Other RFLP probes gave rise to 2–5 loci, inferring that B. rapa genome duplication is a general phenomenon through 10 chromosomes. The map includes five loci of FLC paralogues, which represent the previously reported BrFLC-1, -2, -3, and -5 and additionally identified BrFLC3 paralogues derived from local segmental duplication on R3.




This article has been cited by other articles:


Home page
 Plant Physiol.Home page
M. R. Broadley, J. P. Hammond, G. J. King, D. Astley, H. C. Bowen, M. C. Meacham, A. Mead, D. A.C. Pink, G. R. Teakle, R. M. Hayden, et al.
Shoot Calcium and Magnesium Concentrations Differ between Subtaxa, Are Highly Heritable, and Associate with Potentially Pleiotropic Loci in Brassica oleracea
Plant Physiology, April 1, 2008; 146(4): 1707 - 1720.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
P. Lou, J. Zhao, J. S. Kim, S. Shen, D. P. Del Carpio, X. Song, M. Jin, D. Vreugdenhil, X. Wang, M. Koornneef, et al.
Quantitative trait loci for flowering time and morphological traits in multiple populations of Brassica rapa
J. Exp. Bot., November 28, 2007; (2007) erm255v1.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
M. E. Schranz, A. J. Windsor, B.-h. Song, A. Lawton-Rauh, and T. Mitchell-Olds
Comparative Genetic Mapping in Boechera stricta, a Close Relative of Arabidopsis
Plant Physiology, May 1, 2007; 144(1): 286 - 298.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2006 by the Genetics Society of America.