help button home button Genetics Email Content Delivery
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Originally published as Genetics Published Articles Ahead of Print on May 15, 2006.

Genetics, Vol. 173, 1851-1869, August 2006, Copyright © 2006
doi:10.1534/genetics.105.049619

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
genetics.105.049619v1
173/4/1851    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Pelosi, L.
Right arrow Articles by Schneider, D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Pelosi, L.
Right arrow Articles by Schneider, D.

Parallel Changes in Global Protein Profiles During Long-Term Experimental Evolution in Escherichia coli

Ludovic Pelosi*, Lauriane Kühn{dagger}, Dorian Guetta*, Jérôme Garin{dagger}, Johannes Geiselmann*, Richard E. Lenski{ddagger} and Dominique Schneider*,1

* Laboratoire Adaptation et Pathogénie des Microorganismes, Université Joseph Fourier, CNRS UMR 5163, 38041 Grenoble, France, {dagger} Laboratoire Chimie des Proteines DRDC-CP, ERM 0201 CEA/INSERM/UJF, 38054 Grenoble, France and {ddagger} Department of Microbiology and Molecular Genetics, Michigan State University, East Lansing, Michigan 48824

1 Corresponding author: Laboratoire Adaptation et Pathogénie des Microorganismes, Université Joseph Fourier, CNRS UMR 5163, Institut Jean Roget, Domaine de la Merci, La Tronche 38700, France.
E-mail: dominique.schneider{at}ujf-grenoble.fr

Twelve populations of Escherichia coli evolved in and adapted to a glucose-limited environment from a common ancestor. We used two-dimensional protein electrophoresis to compare two evolved clones, isolated from independently derived populations after 20,000 generations. Exceptional parallelism was detected. We compared the observed changes in protein expression profiles with previously characterized global transcription profiles of the same clones; this is the first time such a comparison has been made in an evolutionary context where these changes are often quite subtle. The two methodologies exhibited some remarkable similarities that highlighted two different levels of parallel regulatory changes that were beneficial during the evolution experiment. First, at the higher level, both methods revealed extensive parallel changes in the same global regulatory network, reflecting the involvement of beneficial mutations in genes that control the ppGpp regulon. Second, both methods detected expression changes of identical gene sets that reflected parallel changes at a lower level of gene regulation. The protein profiles led to the discovery of beneficial mutations affecting the malT gene, with strong genetic parallelism across independently evolved populations. Functional and evolutionary analyses of these mutations revealed parallel phenotypic decreases in the maltose regulon expression and a high level of polymorphism at this locus in the evolved populations.




This article has been cited by other articles:


Home page
 Proc. Natl. Acad. Sci. USAHome page
Z. D. Blount, C. Z. Borland, and R. E. Lenski
Inaugural Article: Historical contingency and the evolution of a key innovation in an experimental population of Escherichia coli
PNAS, June 10, 2008; 105(23): 7899 - 7906.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
L. Yang, J. A. J. Haagensen, L. Jelsbak, H. K. Johansen, C. Sternberg, N. Hoiby, and S. Molin
In Situ Growth Rates and Biofilm Development of Pseudomonas aeruginosa Populations in Chronic Lung Infections
J. Bacteriol., April 15, 2008; 190(8): 2767 - 2776.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
M. Le Gac, M. D. Brazas, M. Bertrand, J. G. Tyerman, C. C. Spencer, R. E. W. Hancock, and M. Doebeli
Metabolic Changes Associated With Adaptive Diversification in Escherichia coli
Genetics, February 1, 2008; 178(2): 1049 - 1060.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
J. T. Witten, C. T. L. Chen, and B. A. Cohen
Complex Genetic Changes in Strains of Saccharomyces cerevisiae Derived by Selection in the Laboratory
Genetics, September 1, 2007; 177(1): 449 - 456.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2006 by the Genetics Society of America.