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Originally published as Genetics Published Articles Ahead of Print on April 19, 2006.
Genetics, Vol. 173, 1571-1583, July 2006, Copyright © 2006
doi:10.1534/genetics.106.056853
Partitioning of the Maize Epigenome by the Number of Methyl Groups on Histone H3 Lysines 9 and 27
Jinghua Shi* and
R. Kelly Dawe*,
,1
* Department of Plant Biology and Department of Genetics, University of Georgia, Athens, Georgia 30602
1 Corresponding author: Department of Plant Biology, Miller Plant Sciences Bldg., University of Georgia, Athens, GA 30602.
E-mail: kelly{at}dogwood.botany.uga.edu
We report a detailed analysis of maize chromosome structure with respect to seven histone H3 methylation states (dimethylation at lysine 4 and mono-, di-, and trimethylation at lysines 9 and 27). Three-dimensional light microscopy and the fine cytological resolution of maize pachytene chromosomes made it possible to compare the distribution of individual histone methylation events to each other and to DNA staining intensity. Major conclusions are that (1) H3K27me2 marks classical heterochromatin; (2) H3K4me2 is limited to areas between and around H3K27me2-marked chromomeres, clearly demarcating the euchromatic gene space; (3) H3K9me2 is restricted to the euchromatic gene space; (4) H3K27me3 occurs in a few (roughly seven) focused euchromatic domains; (5) centromeres and CENP-C are closely associated with H3K9me2 and H3K9me3; and (6) histone H4K20 di- and trimethylation are nearly or completely absent in maize. Each methylation state identifies different regions of the epigenome. We discuss the evolutionary lability of histone methylation profiles and draw a distinction between H3K9me2-mediated gene silencing and heterochromatin formation.
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