- THIS ARTICLE
- Full Text
- Full Text (PDF)
-
All Versions of this Article:
genetics.106.056721v1
173/3/1275 most recent - Alert me when this article is cited
- Alert me if a correction is posted
- SERVICES
- Email this article to a friend
- Similar articles in this journal
- Similar articles in PubMed
- Alert me to new issues of the journal
- Download to citation manager
- Reprints & Permissions
- CITING ARTICLES
- Citing Articles via HighWire
- Citing Articles via Google Scholar
- GOOGLE SCHOLAR
- Articles by Bell, L. R.
- Articles by Herman, R. K.
- Search for Related Content
- PUBMED
- PubMed Citation
- Articles by Bell, L. R.
- Articles by Herman, R. K.
Originally published as Genetics Published Articles Ahead of Print on April 30, 2006.
Genetics, Vol. 173, 1275-1286, July 2006, Copyright © 2006
doi:10.1534/genetics.106.056721
The Molecular Identities of the Caenorhabditis elegans Intraflagellar Transport Genes dyf-6, daf-10 and osm-1
Leslie R. Bell1, Steven Stone2, John Yochem, Jocelyn E. Shaw and Robert K. Herman
Department of Genetics, Cell Biology, and Development, University of Minnesota, Minneapolis, Minnesota 55455
1 Corresponding author: Department of Genetics, Cell Biology, and Development, University of Minnesota, Room 6-160 Jackson Hall, 321 Church Street SE, Minneapolis, MN 55455.
E-mail: bellx035{at}umn.edu
The Caenorhabditis elegans genes dyf-6, daf-10, and osm-1 are among the set of genes that affect chemotaxis and the ability of certain sensory neurons to take up fluorescent dyes from the environment. Some genes in this category are known to be required for intraflagellar transport (IFT), which is the bidirectional movement of raft-like particles along the axonemes of cilia and flagella. The cloning of dyf-6, daf-10, and osm-1 are described here. The daf-10 and osm-1 gene products resemble each other and contain WD and WAA repeats. DYF-6, the product of a complex locus, lacks known motifs, but orthologs are present in flies and mammals. Phenotypic analysis of dyf-6 mutants expressing an OSM-6::GFP reporter indicates that the cilia of the amphid and phasmid dendritic endings are foreshortened. Consistent with genetic mosaic analysis, which indicates that dyf-6 functions in neurons of the amphid sensilla, DYF-6::GFP is expressed in amphid and phasmid neurons. Movement of DYF-6::GFP within the ciliated endings of the neurons indicates that DYF-6 is involved in IFT. In addition, IFT can be observed in dauer larvae.
This article has been cited by other articles:
 |
|
 |
|
  C.-C. Tsao and M. A. Gorovsky Different Effects of Tetrahymena IFT172 Domains on Anterograde and Retrograde Intraflagellar Transport Mol. Biol. Cell, April 1, 2008; 19(4): 1450 - 1461. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Absalon, T. Blisnick, L. Kohl, G. Toutirais, G. Dore, D. Julkowska, A. Tavenet, and P. Bastin Intraflagellar Transport and Functional Analysis of Genes Required for Flagellum Formation in Trypanosomes Mol. Biol. Cell, March 1, 2008; 19(3): 929 - 944. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Bacaj, Y. Lu, and S. Shaham The Conserved Proteins CHE-12 and DYF-11 Are Required for Sensory Cilium Function in Caenorhabditis elegans Genetics, February 1, 2008; 178(2): 989 - 1002. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Kunitomo and Y. Iino Caenorhabditis elegans DYF-11, an orthologue of mammalian Traf3ip1/MIP-T3, is required for sensory cilia formation. Genes Cells, January 1, 2008; 13(1): 13 - 25. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Gouttenoire, U. Valcourt, C. Bougault, E. Aubert-Foucher, E. Arnaud, L. Giraud, and F. Mallein-Gerin Knockdown of the Intraflagellar Transport Protein IFT46 Stimulates Selective Gene Expression in Mouse Chondrocytes and Affects Early Development in Zebrafish J. Biol. Chem., October 19, 2007; 282(42): 30960 - 30973. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Bacaj and S. Shaham Temporal Control of Cell-Specific Transgene Expression in Caenorhabditis elegans Genetics, August 1, 2007; 176(4): 2651 - 2655. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Kobayashi, K. Gengyo-Ando, T. Ishihara, I. Katsura, and S. Mitani IFT-81 and IFT-74 are required for intraflagellar transport in C. elegans Genes Cells, May 1, 2007; 12(5): 593 - 602. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Ou, M. Koga, O. E. Blacque, T. Murayama, Y. Ohshima, J. C. Schafer, C. Li, B. K. Yoder, M. R. Leroux, and J. M. Scholey Sensory Ciliogenesis in Caenorhabditis elegans: Assignment of IFT Components into Distinct Modules Based on Transport and Phenotypic Profiles Mol. Biol. Cell, May 1, 2007; 18(5): 1554 - 1569. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Hou, H. Qin, J. A. Follit, G. J. Pazour, J. L. Rosenbaum, and G. B. Witman Functional analysis of an individual IFT protein: IFT46 is required for transport of outer dynein arms into flagella J. Cell Biol., February 26, 2007; 176(5): 653 - 665. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y.-K. Bae, H. Qin, K. M. Knobel, J. Hu, J. L. Rosenbaum, and M. M. Barr General and cell-type specific mechanisms target TRPP2/PKD-2 to cilia Development, October 1, 2006; 133(19): 3859 - 3870. [Abstract] [Full Text] [PDF]
|
|