help button home button Genetics J Clin Inv
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS

Originally published as Genetics Published Articles Ahead of Print on April 2, 2006.

Genetics, Vol. 173, 1007-1021, June 2006, Copyright © 2006
doi:10.1534/genetics.105.053165

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Data Supplement
Right arrow All Versions of this Article:
genetics.105.053165v1
173/2/1007    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lamb, J. C.
Right arrow Articles by Birchler, J. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lamb, J. C.
Right arrow Articles by Birchler, J. A.

Retroelement Genome Painting: Cytological Visualization of Retroelement Expansions in the Genera Zea and Tripsacum

Jonathan C. Lamb and James A. Birchler1

Division of Biological Sciences, University of Missouri, Columbia, Missouri 65211-7400

1 Corresponding author: University of Missouri, 117 Tucker Hall, Columbia, MO 65211-7400.
E-mail: birchlerj{at}missouri.edu

Divergence of abundant genomic elements among the Zea and Tripsacum genera was examined cytologically and a tool kit established for subsequent studies. The LTR regions from the CRM, Huck, Grande, Prem1, Prem2/Ji, Opie, Cinful-1, and Tekay retroelement families were used as FISH probes on mitotic chromosome spreads from a "trispecies" hybrid containing chromosomes from each of three species: Zea mays (2n = 20), Z. diploperennis (2n = 20), and Tripsacum dactyloides (2n = 36). Except for Tekay, which painted both Zea and Tripsacum chromosomes with nearly equal intensity, the retroelement probes hybridized strongly to the Zea chromosomes, allowing them to be distinguished from those of Tripsacum. Huck and Grande hybridized more intensely to maize than to Z. diploperennis chromosomes. Tripsacum genomic clones containing retroelement sequences were isolated that specifically paint Tripsacum chromosomes. The retroelement paints proved effective for distinguishing different genomes in interspecific hybrids and visualizing alien chromatin from T. dactyloides introgressed into maize lines. Other FISH probes (180-bp knob, TR-1, 5S, NOR, Cent4, CentC, rp1, rp3, and {alpha}-ZeinA) could be simultaneously visualized with the retroelement probes, emphasizing the value of the retroelement probes for cytogenetic studies of Zea and Tripsacum.




This article has been cited by other articles:


Home page
GeneticsHome page
D.-H. Koo and J. Jiang
Extraordinary Tertiary Constrictions of Tripsacum dactyloides Chromosomes: Implications for Karyotype Evolution of Polyploids Driven by Segmental Chromosome Losses
Genetics, June 1, 2008; 179(2): 1119 - 1123.
[Abstract] [Full Text] [PDF]


Home page
GeneticsHome page
F. I. E. Amarillo and H. W. Bass
A Transgenomic Cytogenetic Sorghum (Sorghum propinquum) Bacterial Artificial Chromosome Fluorescence in Situ Hybridization Map of Maize (Zea mays L.) Pachytene Chromosome 9, Evidence for Regions of Genome Hyperexpansion
Genetics, November 1, 2007; 177(3): 1509 - 1526.
[Abstract] [Full Text] [PDF]


Home page
GeneticsHome page
J. C. Lamb, T. Danilova, M. J. Bauer, J. M. Meyer, J. J. Holland, M. D. Jensen, and J. A. Birchler
Single-Gene Detection and Karyotyping Using Small-Target Fluorescence in Situ Hybridization on Maize Somatic Chromosomes
Genetics, March 1, 2007; 175(3): 1047 - 1058.
[Abstract] [Full Text] [PDF]




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2006 by the Genetics Society of America.