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Originally published as Genetics Published Articles Ahead of Print on March 1, 2006.
Genetics, Vol. 173, 287-296, May 2006, Copyright © 2006
doi:10.1534/genetics.105.053256
A Genetic and Cytogenetic Map for the Duck (Anas platyrhynchos)
Yinhua Huang*,
Yonghui Zhao*,
Chris S. Haley
,
Shengqiang Hu
,
Jinping Hao,
Changxin Wu
and
Ning Li*,1
* State Key Laboratory for Agrobiotechnology, China Agricultural University, Beijing 100094, People's Republic of China, Roslin Institute, Roslin, Midlotthian EH25 9PS, United Kingdom, Gold Star Duck Production, Beijing 100076, People's Republic of China and College of Animal Science and Technology, China Agricultural University, Beijing 100094, People's Republic of China
1 Corresponding author: State Key Laboratory for Agrobiotechnology, China Agricultural University, Yuanmingyuan, West Rd. 2, Haidian District, Beijing 100094, People's Republic of China.
E-mail: ninglbau{at}public3.bta.net.cn
A genetic linkage map for the duck (Anas platyrhynchos) was developed within a cross between two extreme Peking duck lines by linkage analysis of 155 polymorphic microsatellite markers, including 84 novel markers reported in this study. A total of 115 microsatellite markers were placed into 19 linkage groups. The sex-averaged map spans 1353.3 cM, with an average interval distance of 15.04 cM. The male map covers 1415 cM, whereas the female map covers only 1387.6 cM. All of the flanking sequences of the 155 polymorphic loci—44 monomorphic loci and a further 41 reported microsatellite loci for duck—were blasted against the chicken genomic sequence, and corresponding orthologs were found for 49. To integrate the genetic and cytogenetic map of the duck genome, 28 BAC clones were screened from a chicken BAC library using the specific PCR primers and localized to duck chromosomes by FISH, respectively. Of 28 BAC clones, 24 were detected definitely on duck chromosomes. Thus, 11 of 19 linkage groups were localized to 10 duck chromosomes. This genetic and cytogenetic map will be helpful for the mapping QTL in duck for breeding applications and for conducting genomic comparisons between chicken and duck.