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Originally published as Genetics Published Articles Ahead of Print on March 17, 2006.
Genetics, Vol. 173, 279-286, May 2006, Copyright © 2006
doi:10.1534/genetics.106.055558
Characterization of the split ends-Like Gene spenito Reveals Functional Antagonism Between SPOC Family Members During Drosophila Eye Development
Jennifer Jemc* and
Ilaria Rebay*,
,
,1
* Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02142, Whitehead Institute for Biomedical Research, Cambridge, Massachusetts 02142 and University of Chicago, Ben May Institute for Cancer Research, Chicago, Illinois 60637
1 Corresponding author: University of Chicago, Ben May Institute for Cancer Research, 927 E. 57th St., Chicago, IL 60637.
E-mail: irebay{at}uchicago.edu
The novel family of SPOC domain proteins is composed of broadly conserved nuclear factors that fall into two subclasses, termed large and small, based on protein size. Members of the large subgroup, which includes Drosophila SPEN and human SHARP, have been characterized as transcriptional corepressors acting downstream of a variety of essential cell signaling pathways, while those of the small subclass have remained largely unstudied. Since SPEN has been implicated in Drosophila eye development, and the small SPOC protein NITO is also expressed in the developing eye, we have used this context to perform a structure–function analysis of NITO and to examine the relationship between the two SPOC family subclasses. Our results demonstrate that the phenotypes obtained from overexpressing NITO share striking similarity to those associated with loss of spen. Dosage-sensitive genetic interactions further support a model of functional antagonism between NITO and SPEN during Drosophila eye development. These results suggest that large and small SPOC family proteins may have opposing functions in certain developmental contexts.
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