Originally published as Genetics Published Articles Ahead of Print on March 1, 2006.

Genetics, Vol. 173, 255-266, May 2006, Copyright © 2006
doi:10.1534/genetics.106.056341

jing Is Required for Wing Development and to Establish the Proximo-Distal Axis of the Leg in Drosophila melanogaster

Joaquim Culi*,{dagger}, Pilar Aroca{dagger},1, Juan Modolell{dagger} and Richard S. Mann*,2

* Department of Biochemistry and Molecular Biophysics, Columbia University, New York, New York 10032 and {dagger} Centro de Biología Molecular Severo Ochoa, CSIC and Universidad Autónoma de Madrid, 28049 Madrid, Spain

2 Corresponding author: Columbia University, 701 W. 168th St., HHSC 1104, New York, NY 10032.
E-mail: rsm10{at}columbia.edu

The establishment of the proximo-distal (PD) axis in the legs of Drosophila melanogaster requires the expression of a nested set of transcription factors that are activated in discreet domains by secreted signaling molecules. The precise regulation of these transcription factor domains is critical for generating the stereotyped morphological characteristics that exist along the PD axis, such as the positioning of specific bristle types and leg joints. Here we provide evidence that the Zn-finger protein encoded by the gene jing is critical for PD axis formation in the Drosophila legs. Our data suggest that jing represses transcription and that it is necessary to keep the proximal gene homothorax (hth) repressed in the medial domain of the PD axis. We further show that jing is also required for alula and vein development in the adult wing. In the wing, Jing is required to repress another proximal gene, teashirt (tsh), in a small domain that will give rise to the alula. Interestingly, we also demonstrate that two other genes affecting alula development, Alula and elbow, also exhibit tsh derepression in the same region of the wing disc as jing– clones. Finally, we show that jing genetically interacts with several members of the Polycomb (Pc) group of genes during development. Together, our data suggest that jing encodes a transcriptional repressor that may participate in a subset of Pc-dependent activities during Drosophila appendage development.