Originally published as Genetics Published Articles Ahead of Print on March 17, 2006.

Genetics, Vol. 173, 163-175, May 2006, Copyright © 2006
doi:10.1534/genetics.106.055822

The Adaptor Protein soc-1/Gab1 Modifies Growth Factor Receptor Output in Caenorhabditis elegans

School of Biological Sciences, University of Southampton, Southampton SO16 7PX, United Kingdom

1 Address for correspondence: School of Biological Sciences, University of Southampton, Bassett Crescent East, Southampton SO16 7PX, United Kingdom. 
E-mail: nah2{at}soton.ac.uk

Previous genetic analysis has shown that dos/soc-1/Gab1 functions positively in receptor tyrosine kinase (RTK)-stimulated Ras/Map kinase signaling through the recruitment of csw/ptp-2/Shp2. Using sensitized assays in Caenorhabditis elegans for let-23/Egfr and daf-2/InsR (insulin receptor-like) signaling, it is shown that soc-1/Gab1 inhibits phospholipase C-{gamma} (PLC{gamma}) and phosphatidylinositol 3'-kinase (PI3K)-mediated signaling. Furthermore, as well as stimulating Ras/Map kinase signaling, soc-1/Gab1 stimulates a poorly defined signaling pathway that represses class 2 daf-2 phenotypes. In addition, it is shown that SOC-1 binds the C-terminal SH3 domain of SEM-5. This binding is likely to be functional as the sem-5(n2195)G201R mutation, which disrupts SOC-1 binding, behaves in a qualitatively similar manner to a soc-1 null allele in all assays for let-23/Egfr and daf-2/InsR signaling that were examined. Further genetic analysis suggests that ptp-2/Shp2 mediates the negative function of soc-1/Gab1 in PI3K-mediated signaling, as well as the positive function in Ras/Map kinase signaling. Other effectors of soc-1/Gab1 are likely to inhibit PLC{gamma}-mediated signaling and stimulate the poorly defined signaling pathway that represses class 2 daf-2 phenotypes. Thus, the recruitment of soc-1/Gab1, and its effectors, into the RTK-signaling complex modifies the cellular response by enhancing Ras/Map kinase signaling while inhibiting PI3K and PLC{gamma}-mediated signaling.




This article has been cited by other articles:


Home page
DevelopmentHome page
K. L. Schmidt, N. Marcus-Gueret, A. Adeleye, J. Webber, D. Baillie, and E. G. Stringham
The cell migration molecule UNC-53/NAV2 is linked to the ARP2/3 complex by ABI-1
Development, February 15, 2009; 136(4): 563 - 574.
[Abstract] [Full Text] [PDF]


Home page
GeneticsHome page
D. S. Patel, A. Garza-Garcia, M. Nanji, J. J. McElwee, D. Ackerman, P. C. Driscoll, and D. Gems
Clustering of Genetically Defined Allele Classes in the Caenorhabditis elegans DAF-2 Insulin/IGF-1 Receptor
Genetics, February 1, 2008; 178(2): 931 - 946.
[Abstract] [Full Text] [PDF]


Home page
DevelopmentHome page
A.-F. Ruaud and J.-L. Bessereau
The P-type ATPase CATP-1 is a novel regulator of C. elegans developmental timing that acts independently of its predicted pump function
Development, March 1, 2007; 134(5): 867 - 879.
[Abstract] [Full Text] [PDF]