- THIS ARTICLE
- Full Text
- Full Text (PDF)
-
All Versions of this Article:
genetics.105.055145v1
172/4/2515 most recent - Alert me when this article is cited
- Alert me if a correction is posted
- SERVICES
- Similar articles in this journal
- Similar articles in PubMed
- Alert me to new issues of the journal
- Download to citation manager
- Reprints & Permissions
- CITING ARTICLES
- Citing Articles via HighWire
- Citing Articles via Google Scholar
- GOOGLE SCHOLAR
- Articles by Lloyd, R. E.
- Articles by St. John, J. C.
- Search for Related Content
- PUBMED
- PubMed Citation
- Articles by Lloyd, R. E.
- Articles by St. John, J. C.
Originally published as Genetics Published Articles Ahead of Print on February 1, 2006.
Genetics, Vol. 172, 2515-2527, April 2006, Copyright © 2006
doi:10.1534/genetics.105.055145
Aberrant Nucleo-cytoplasmic Cross-Talk Results in Donor Cell mtDNA Persistence in Cloned Embryos
Rhiannon E. Lloyd*,1,
Joon-Hee Lee
,1,
Ramiro Alberio,
Emma J. Bowles*,
João Ramalho-Santos
,
Keith H. S. Campbell and
Justin C. St. John*,2
* The Mitochondrial and Reproductive Genetics Group, The Medical School, The University of Birmingham, Birmingham B15 2TT, United Kingdom, Animal Development and Biotechnology Group, University of Nottingham, School of Biosciences, Sutton Bonington, Loughborough, Leics LE12 5RD, United Kingdom and Center for Neuroscience and Cell Biology, Department of Zoology, University of Coimbra, 3004-517 Coimbra, Portugal
2 Corresponding author: Mitochondrial and Reproductive Genetics Group, Room EF20A, The Medical School, The University of Birmingham, Edgbaston, Birmingham B15 2TT, United Kingdom.
E-mail: j.stjohn.1{at}bham.ac.uk
Mitochondrial DNA is an extranuclear genome normally maternally inherited through the oocyte. However, the use of nuclear transfer can result in both donor cell and recipient oocyte mitochondrial DNA persisting through to blastocyst and being transmitted to the offspring. The degree of donor mitochondrial DNA transmission appears to be random and currently no evidence exists to explain this phenomenon. To determine whether this is a dilution factor or directly related to the transcriptional status of the donor cell in respect of mitochondrial DNA transcription factors, we have generated sheep nuclear transfer embryos using donor cells: (1) possessing their full mitochondrial DNA complement, (2) those partially depleted, and (3) those depleted but containing residual levels. For each donor type, donor mitochondrial DNA persisted in some blastocysts. It is evident from the donor cells used that nuclear-encoded mitochondrial DNA transcription and replication factors persist even after mitochondrial DNA depletion, as do transcripts for some of the mitochondrial-encoded genes. These cells are therefore still programmed to drive mitochondrial DNA replication and transcription. In nuclear transfer-derived embryos, we have observed the persistence of these nuclear-encoded mitochondrial DNA transcription and replication factors but not in those embryos generated through in vitro fertilization. Consequently, nucleo-mitochondrial interaction following nuclear transfer is out of sequence as the onset of mitochondrial replication is a postimplantation event.
This article has been cited by other articles:
 |
|
 |
|
  E. J. Bowles, R. T. Tecirlioglu, A. J. French, M. K. Holland, and J. C. St. John Mitochondrial DNA Transmission and Transcription After Somatic Cell Fusion to One or More Cytoplasts Stem Cells, March 1, 2008; 26(3): 775 - 782. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. M. Facucho-Oliveira, J. Alderson, E. C. Spikings, S. Egginton, and J. C. St. John Mitochondrial DNA replication during differentiation of murine embryonic stem cells J. Cell Sci., November 15, 2007; 120(22): 4025 - 4034. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. J. Bowles, J.-H. Lee, R. Alberio, R. E. I. Lloyd, D. Stekel, K. H. S. Campbell, and J. C. St. John Contrasting Effects of in Vitro Fertilization and Nuclear Transfer on the Expression of mtDNA Replication Factors Genetics, July 1, 2007; 176(3): 1511 - 1526. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Amaral, J. Ramalho-Santos, and J. C. St John The expression of polymerase gamma and mitochondrial transcription factor A and the regulation of mitochondrial DNA content in mature human sperm Hum. Reprod., June 1, 2007; 22(6): 1585 - 1596. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. C. Spikings, J. Alderson, and J. C. St. John Regulated Mitochondrial DNA Replication During Oocyte Maturation Is Essential for Successful Porcine Embryonic Development Biol Reprod, February 1, 2007; 76(2): 327 - 335. [Abstract] [Full Text] [PDF]
|
|