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Originally published as Genetics Published Articles Ahead of Print on February 1, 2006.

Genetics, Vol. 172, 2391-2403, April 2006, Copyright © 2006
doi:10.1534/genetics.105.052829

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Efficient Gene Targeting in Drosophila With Zinc-Finger Nucleases

Kelly Beumer, Gargi Bhattacharyya1, Marina Bibikova2, Jonathan K. Trautman and Dana Carroll3

Department of Biochemistry, University of Utah School of Medicine, Salt Lake City, Utah 84112

3 Corresponding author: Department of Biochemistry, University of Utah School of Medicine, 15 North Medical Drive East, Room 4100, Salt Lake City, UT 84112-5650.
E-mail: dana{at}biochem.utah.edu

This report describes high-frequency germline gene targeting at two genomic loci in Drosophila melanogaster, y and ry. In the best case, nearly all induced parents produced mutant progeny; 25% of their offspring were new mutants and most of these were targeted gene replacements resulting from homologous recombination (HR) with a marked donor DNA. The procedure that generates these high frequencies relies on cleavage of the target by designed zinc-finger nucleases (ZFNs) and production of a linear donor in situ. Increased induction of ZFN expression led to higher frequencies of gene targeting, demonstrating the beneficial effect of activating the target. In the absence of a homologous donor DNA, ZFN cleavage led to the recovery of new mutants at three loci—y, ry and bw—through nonhomologous end joining (NHEJ) after cleavage. Because zinc fingers can be directed to a broad range of DNA sequences and targeting is very efficient, this approach promises to allow genetic manipulation of many different genes, even in cases where the mutant phenotype cannot be predicted.




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