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Genetics, Vol. 172, 1545-1555, March 2006, Copyright © 2006
doi:10.1534/genetics.105.051490
Nonallelic Interactions Between het-c and a Polymorphic Locus, pin-c, Are Essential for Nonself Recognition and Programmed Cell Death in Neurospora crassa
Isao Kaneko1, Karine Dementhon, Qijun Xiang2 and N. Louise Glass3
Department of Plant and Microbial Biology, University of California, Berkeley, California 94720
3 Corresponding author: Department of Plant and Microbial Biology, 111 Koshland Hall, 3102, University of California, Berkeley, CA 94720-3102.
E-mail: lglass{at}nature.berkeley.edu
Nonself recognition in filamentous fungi is conferred by genetic differences at het (heterokaryon incompatibility) loci. When individuals that differ in het specificity undergo hyphal fusion, the heterokaryon undergoes a programmed cell death reaction or is highly unstable. In Neurospora crassa, three allelic specificities at the het-c locus are conferred by a highly polymorphic domain. This domain shows trans-species polymorphisms indicative of balancing selection, consistent with the role of het loci in nonself recognition. We determined that a locus closely linked to het-c, called pin-c (partner for incompatibility with het-c) was required for het-c nonself recognition and heterokaryon incompatibility (HI). The pin-c alleles in isolates that differ in het-c specificity were extremely polymorphic. Heterokaryon and transformation tests showed that nonself recognition was mediated by synergistic nonallelic interactions between het-c and pin-c, while allelic interactions at het-c increased the severity of the HI phenotype. The pin-c locus encodes a protein containing a HET domain; predicted proteins containing HET domains are frequent in filamentous ascomycete genomes. These data suggest that nonallelic interactions may be important in nonself recognition in filamentous fungi and that proteins containing a HET domain may be a key factor in these interactions.
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