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Originally published as Genetics Published Articles Ahead of Print on October 11, 2005.
Genetics, Vol. 172, 929-942, February 2006, Copyright © 2006
doi:10.1534/genetics.104.035550
The CRAL/TRIO and GOLD Domain Protein CGR-1 Promotes Induction of Vulval Cell Fates in Caenorhabditis elegans and Interacts Genetically With the Ras Signaling Pathway
Jessica L. Goldstein*,
Danielle Glossip*,
Sudhir Nayak
and
Kerry Kornfeld*,1
* Department of Molecular Biology and Pharmacology and Department of Genetics, Washington University School of Medicine, St. Louis, Missouri 63110
1 Corresponding author: Department of Molecular Biology and Pharmacology, Washington University School of Medicine, Campus Box 8103, 660 S. Euclid Ave., St. Louis, MO 63110.
E-mail: kornfeld{at}wustl.edu
Ras-mediated signaling is necessary for the induction of vulval cell fates during Caenorhabditis elegans development. We identified cgr-1 by screening for suppressors of the ectopic vulval cell fates caused by a gain-of-function mutation of the let-60 ras gene. Analysis of two cgr-1 loss-of-function mutations indicates that cgr-1 positively regulates induction of vulval cell fates. cgr-1 is likely to function at a step in the Ras signaling pathway that is downstream of let-60, which encodes Ras, and upstream of lin-1, which encodes a transcription factor, if these genes function in a linear signaling pathway. These genetic studies are also consistent with the model that cgr-1 functions in a parallel pathway that promotes vulval cell fates. Localized expression studies suggest that cgr-1 functions cell autonomously to affect vulval cell fates. cgr-1 also functions early in development, since cgr-1 is necessary for larval viability. CGR-1 contains a CRAL/TRIO domain likely to bind a small hydrophobic ligand and a GOLD domain that may mediate interactions with proteins. A bioinformatic analysis revealed that there is a conserved family of CRAL/TRIO and GOLD domain-containing proteins that includes members from vertebrates and Drosophila. The analysis of cgr-1 identifies a novel in vivo function for a member of this family and a potential new regulator of Ras-mediated signaling.
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