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Originally published as Genetics Published Articles Ahead of Print on December 1, 2005.
Genetics, Vol. 172, 1359-1362, February 2006, Copyright © 2006
doi:10.1534/genetics.105.051144
Chromosomal Fragmentation Is the Major Consequence of the rdgB Defect in Escherichia coli
Lisa Lukas and Andrei Kuzminov1
Department of Microbiology, University of Illinois, Urbana, Illinois 61801
1 Corresponding author: University of Illinois, B103 C&LSL, 601 S. Goodwin Ave., Urbana, IL 61801-3709.
E-mail: kuzminov{at}life.uiuc.edu
The rdgB mutants depend on recombinational repair of double-strand breaks. To assess other consequences of rdgB inactivation in Escherichia coli, we isolated RdgB-dependent mutants. All transposon inserts making cells dependent on RdgB inactivate genes of double-strand break repair, indicating that chromosomal fragmentation is the major consequence of RdgB inactivation.
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