Originally published as Genetics Published Articles Ahead of Print on December 1, 2005.

Genetics, Vol. 172, 1277-1285, February 2006, Copyright © 2006
doi:10.1534/genetics.105.049908

Transcriptome Analysis and Physical Mapping of Barley Genes in Wheat–Barley Chromosome Addition Lines

Seungho Cho*, David F. Garvin*,{dagger} and Gary J. Muehlbauer*,1

* Department of Agronomy and Plant Genetics, University of Minnesota, St. Paul, Minnesota 55108 and {dagger} Plant Science Research Unit, United States Department of Agriculture-Agricultural Research Service, St. Paul, Minnesota 55108

1 Corresponding author: Department of Agronomy and Plant Genetics, University of Minnesota, 411 Borlaug Hall, St. Paul, MN 55108.
E-mail: muehl003{at}umn.edu

Wheat–barley chromosome addition lines are useful genetic resources for a variety of studies. In this study, transcript accumulation patterns in Betzes barley, Chinese Spring wheat, and Chinese Spring–Betzes chromosome addition lines were examined with the Barley1 Affymetrix GeneChip probe array. Of the 4014 transcripts detected in Betzes but not in Chinese Spring, 365, 271, 265, 323, 194, and 369 were detected in wheat–barley disomic chromosome addition lines 2(2H), 3(3H), 4(4H), 7(5H), 6(6H), and 1(7H), respectively. Thus, 1787 barley transcripts were detected in a wheat genetic background and, by virtue of the addition line in which they were detected, were physically mapped to barley chromosomes. We validated and extended our approach to physically map barley genes to the long and short arms of chromosome 6(6H). Our physical map data exhibited a high level of synteny with homologous sequences on the wheat and/or rice syntenous chromosomes, indicating that our barley physical maps are robust. Our results show that barley transcript detection in wheat–barley chromosome addition lines is an efficient approach for large-scale physical mapping of genes.