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Originally published as Genetics Published Articles Ahead of Print on December 1, 2005.
Genetics, Vol. 172, 1263-1275, February 2006, Copyright © 2006
doi:10.1534/genetics.105.051219
Structural Diversity and Differential Transcription of the Patatin Multicopy Gene Family During Potato Tuber Development
Robert M. Stupar*,
Karen A. Beaubien*,
Weiwei Jin*,
Junqi Song*,
Mi-Kyung Lee
,
Chengcang Wu,
Hong-Bin Zhang,
Bin Han
and
Jiming Jiang*,1
* Department of Horticulture, University of Wisconsin, Madison, Wisconsin 53706, Department of Soil and Crop Sciences, Texas A&M University, College Station, Texas 77843 and National Center for Gene Research, Chinese Academy of Sciences, Shanghai 200233, People's Republic of China
1 Corresponding author: Department of Horticulture, University of Wisconsin, 1575 Linden Dr., Madison, WI 53706.
E-mail: jjiang1{at}wisc.edu
The patatin multicopy gene family encodes the major storage protein in potato tubers and is organized as a single cluster in the potato genome. We sequenced a 154-kb bacterial artificial chromosome (BAC) clone containing a portion of the patatin gene cluster. Two putatively functional patatin genes were found in this BAC. These two genes are embedded within arrays of patatin pseudogenes. Using a chromatin immunoprecipitation method we demonstrate that the dramatic increase of patatin gene expression during the transition from stolons to tubers coincides with an increase of histone H4 lysine acetylation. We used 3' rapid amplification of cDNA ends to profile expression of different patatin genes during tuber development. The profiling results revealed differential expression patterns of specific patatin gene groups throughout six different stages of tuber development. One group of patatin gene transcripts, designated patatin gene group A, was found to be the most abundant group during all stages of tuber development. Other patatin gene groups, with a 48-bp insertion in the 3'-untranslated region, are not expressed in stolons but display a gradual increase in expression level following the onset of tuberization. These results demonstrate that the patatin genes exhibit alterations in chromatin state and differential transcriptional regulation during the developmental transition from stolons into tubers, in which there is an increased demand for protein storage.
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