Originally published as Genetics Published Articles Ahead of Print on September 12, 2005.

Genetics, Vol. 172, 709-711, January 2006, Copyright © 2006
doi:10.1534/genetics.105.047662

Eliminating Gene Conversion Improves High-Throughput Genetics in Saccharomyces cerevisiae

Jewel A. Daniel*, Jiyoun Yoo*, Blaine T. Bettinger{dagger}, David C. Amberg{dagger} and Daniel J. Burke*,1

* Department of Biochemistry and Molecular Genetics, University of Virginia Medical Center, Charlottesville, Virginia 22908-07333 and {dagger} Department of Biochemistry and Molecular Biology, SUNY Upstate Medical University, Syracuse, New York 13210-2339

1 Corresponding author: Department of Biochemistry and Molecular Genetics, University of Virginia Medical Center, 1300 Jefferson Park Ave., Box 800733, Charlottesville, VA 22908-07333.
E-mail: dburke{at}virginia.edu

Synthetic genetic analysis was improved by eliminating leaky expression of the HIS3 reporter and gene conversion between the HIS3 reporter and his3{Delta}1. Leaky expression was eliminated using 3-aminotriazole and gene conversion was eliminated by using the Schizosaccharomyces pombe his5+ gene, resulting in a 5- to 10-fold improvement in the efficiency of SGA.




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