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Originally published as Genetics Published Articles Ahead of Print on September 12, 2005.

Genetics, Vol. 172, 53-65, January 2006, Copyright © 2006
doi:10.1534/genetics.105.046441

Diverse Functions of Spindle Assembly Checkpoint Genes in Saccharomyces cerevisiae

Jewel A. Daniel, Brice E. Keyes, Yvonne P. Y. Ng, C. Onyi Freeman and Daniel J. Burke1

Department of Biochemistry and Molecular Genetics, University of Virginia Medical Center, Charlottesville, Virginia 22908

1 Corresponding author: Department of Biochemistry and Molecular Genetics, University of Virginia Medical Center, 1300 Jefferson Park Ave., Box 800733, Charlottesville, VA 22908.
E-mail: dburke{at}virginia.edu

The spindle assembly checkpoint regulates the metaphase-to-anaphase transition from yeast to humans. We examined the genetic interactions with four spindle assembly checkpoint genes to identify nonessential genes involved in chromosome segregation, to identify the individual roles of the spindle assembly checkpoint genes within the checkpoint, and to reveal potential complexity that may exist. We used synthetic genetic array (SGA) analysis using spindle assembly checkpoint mutants mad1, mad2, mad3, and bub3. We found 228 synthetic interactions with the four spindle assembly checkpoint mutants with substantial overlap in the spectrum of interactions between mad1, mad2, and bub3. In contrast, there were many synthetic interactions that were common to mad1, mad2, and bub3 that were not shared by mad3. We found shared interactions between pairs of spindle assembly checkpoint mutants, suggesting additional complexity within the checkpoint and unique interactions for all of the spindle assembly checkpoint genes. We show that most genes in the interaction network, including ones with unique interactions, affect chromosome transmission or microtubule function, suggesting that the complexity of interactions reflects diverse roles for the checkpoint genes within the checkpoint. Our analysis expands our understanding of the spindle assembly checkpoint and identifies new candidate genes with possible roles in chromosome transmission and mitotic spindle function.




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