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Originally published as Genetics Published Articles Ahead of Print on October 3, 2005.
Genetics, Vol. 172, 293-304, January 2006, Copyright © 2006
doi:10.1534/genetics.105.047035
Triglyceride Pools, Flight and Activity Variation at the Gpdh Locus in Drosophila melanogaster
Thomas J. S. Merritt, Efe Sezgin, Chen-Tseh Zhu and Walter F. Eanes1
Department of Ecology and Evolution, State University of New York, Stony Brook, New York 11794
1 Corresponding author: Department of Ecology and Evolution, State University of New York, Stony Brook, NY 11794.
E-mail: walter{at}life.bio.sunysb.edu
We have created a set of P-element excision-derived Gpdh alleles that generate a range of GPDH activity phenotypes ranging from zero to full activity. By placing these synthetic alleles in isogenic backgrounds, we characterize the effects of minor and major activity variation on two different aspects of Gpdh function: the standing triglyceride pool and glycerol-3-phosphate shuttle-assisted flight. We observe small but statistically significant reductions in triglyceride content for adult Gpdh genotypes possessing 33–80% reductions from normal activity. These small differences scale to a notable proportion of the observed genetic variation in triglyceride content in natural populations. Using a tethered fly assay to assess flight metabolism, we observed that genotypes with 100 and 66% activity exhibited no significant difference in wing-beat frequency (WBF), while activity reductions from 60 to 10% showed statistically significant reductions of 
7% in WBF. These studies show that the molecular polymorphism associated with GPDH activity could be maintained in natural populations by selection in the triglyceride pool.
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