help button home button Genetics J Exp Med
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Originally published as Genetics Published Articles Ahead of Print on September 2, 2005.

Genetics, Vol. 171, 1535-1548, December 2005, Copyright © 2005
doi:10.1534/genetics.105.046144

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Data Supplement
Right arrow All Versions of this Article:
genetics.105.046144v1
171/4/1535    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Decottignies, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Decottignies, A.

Capture of Extranuclear DNA at Fission Yeast Double-Strand Breaks

Anabelle Decottignies1

Cellular Genetics, Christian de Duve Institute of Cellular Pathology, Catholic University of Louvain, 1200 Brussels, Belgium

1 Address for correspondence: Cellular Genetics, Christian de Duve Institute of Cellular Pathology, Catholic University of Louvain, Ave. Hippocrate 74+3, 1200 Brussels, Belgium.
E-mail: anabelle.decottignies{at}gece.ucl.ac.be

Proper repair of DNA double-strand breaks (DSBs) is necessary for the maintenance of genomic integrity. Here, a new simple assay was used to study extrachromosomal DSB repair in Schizosaccharomyces pombe. Strikingly, DSB repair was associated with the capture of fission yeast mitochondrial DNA (mtDNA) at high frequency. Capture of mtDNA fragments required the Lig4p/Pku70p nonhomologous end-joining (NHEJ) machinery and its frequency was highly increased in fission yeast cells grown to stationary phase. The fission yeast Mre11 complex Rad32p/Rad50p/Nbs1p was also required for efficient capture of mtDNA at DSBs, supporting a role for the complex in promoting intermolecular ligation. Competition assays further revealed that microsatellite DNA from higher eukaryotes was preferentially captured at yeast DSBs. Finally, cotransformation experiments indicated that, in NHEJ-deficient cells, capture of extranuclear DNA at DSBs was observed if homologies—as short as 8 bp—were present between DNA substrate and DSB ends. Hence, whether driven by NHEJ, microhomology-mediated end-joining, or homologous recombination, DNA capture associated with DSB repair is a mutagenic process threatening genomic stability.




This article has been cited by other articles:


Home page
 GeneticsHome page
A. Decottignies
Microhomology-Mediated End Joining in Fission Yeast Is Repressed by Pku70 and Relies on Genes Involved in Homologous Recombination
Genetics, July 1, 2007; 176(3): 1403 - 1415.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
B. Pardo, E. Ma, and S. Marcand
Mismatch Tolerance by DNA Polymerase Pol4 in the Course of Nonhomologous End Joining in Saccharomyces cerevisiae
Genetics, April 1, 2006; 172(4): 2689 - 2694.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
A. Kegel, P. Martinez, S. D. Carter, and S. U. Astrom
Genome wide distribution of illegitimate recombination events in Kluyveromyces lactis
Nucleic Acids Res., March 20, 2006; 34(5): 1633 - 1645.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2005 by the Genetics Society of America.