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Originally published as Genetics Published Articles Ahead of Print on August 3, 2005.
Genetics, Vol. 171, 985-1001, November 2005, Copyright © 2005
doi:10.1534/genetics.105.044842
Chromosome Complement of the Fungal Plant Pathogen Fusarium graminearum Based on Genetic and Physical Mapping and Cytological Observations
L. R. Gale*,
J. D. Bryant
,
S. Calvo
,
H. Giese
,
T. Katan**,
K. O'Donnell,
H. Suga,
M. Taga,
T. R. Usgaard,
T. J. Ward and
H. C. Kistler*,,1
* Cereal Disease Laboratory, U.S. Department of Agriculture, Agricultural Research Service, St. Paul, Minnesota 55108, Department of Plant Pathology, University of Minnesota, St. Paul, Minnesota 55108, Broad Institute of MIT and Harvard University, Cambridge, Massachusetts 02141, Department of Ecology, Section of Genetics and Microbiology, Royal Veterinary and Agricultural University, Copenhagen, DK-1871 Frederiksberg C Denmark, ** Volcani Center, 50250 Bet Dagan, Israel, National Center for Agricultural Utilization Research, U.S. Department of Agriculture, Agricultural Research Service, Peoria, Illinois 61604, Life Science Research Center, Division of Genomics Research, Gifu University, Gifu 501-1193, Japan and Department of Biology, Faculty of Science, Okayama University, Okayama 700-8530, Japan
1 Corresponding author: USDA, ARS, Cereal Disease Laboratory, University of Minnesota, 1551 Lindig St., St. Paul, MN 55108.
E-mail: hckist{at}umn.edu
A genetic map of the filamentous fungus Fusarium graminearum (teleomorph: Gibberella zeae) was constructed to both validate and augment the draft whole-genome sequence assembly of strain PH-1. A mapping population was created from a cross between mutants of the sequenced strain (PH-1, NRRL 31084, originally isolated from Michigan) and a field strain from Minnesota (00-676, NRRL 34097). A total of 111 ascospore progeny were analyzed for segregation at 235 loci. Genetic markers consisted of sequence-tagged sites, primarily detected as dCAPS or CAPS (n = 131) and VNTRs (n = 31), in addition to AFLPs (n = 66) and 7 other markers. While most markers exhibited Mendelian inheritance, segregation distortion was observed for 25 predominantly clustered markers. A linkage map was generated using the Kosambi mapping function, using a LOD threshold value of 3.5. Nine linkage groups were detected, covering 1234 cM and anchoring 99.83% of the draft sequence assembly. The nine linkage groups and the 22 anchored scaffolds from the sequence assembly could be assembled into four chromosomes, leaving only five smaller scaffolds (59,630 bp total) of the nuclear DNA unanchored. A chromosome number of four was confirmed by cytological karyotyping. Further analysis of the genetic map data identified variation in recombination rate in different genomic regions that often spanned several hundred kilobases.
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