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Originally published as Genetics Published Articles Ahead of Print on August 22, 2005.
Genetics, Vol. 171, 949-957, November 2005, Copyright © 2005
doi:10.1534/genetics.104.036467
CBT1 Interacts Genetically With CBP1 and the Mitochondrially Encoded Cytochrome b Gene and Is Required to Stabilize the Mature Cytochrome b mRNA of Saccharomyces cerevisiae
Timothy P. Ellis*,1,
Melissa S. Schonauer
and
Carol L. Dieckmann*,,2
* Department of Biochemistry and Molecular Biophysics and Department of Molecular and Cellular Biology, University of Arizona, Tucson, Arizona 85721
2 Corresponding author: Department of Biochemistry and Molecular Biophysics, P.O. Box 210106, University of Arizona, Tucson, AZ 857210106.
E-mail: dieckman{at}u.arizona.edu
Mutation of a CCG sequence in the 5'-untranslated region of the mitochondrially encoded cytochrome b mRNA in Saccharomyces cerevisiae results in destabilization of the message and respiratory deficiency of the mutant strain. This phenotype mimics that of a mutation in the nuclear CBP1 gene. Here it is shown that overexpression of the nuclear CBT1 gene, due to a transposon insertion in the 5'-untranslated region, rescues the respiratory defects resulting from mutating the CCG sequence to ACG. Overexpressing alleles of CBT1 are allelic to soc1, a previously isolated suppressor of cbp1ts-induced temperature sensitivity of respiratory growth. Quantitative primer extension analysis indicated that cbt1 null strains have defects in 5'-end processing of precursor cytochrome b mRNA to the mature form. Cbt1p is also required for stabilizing the mature cytochrome b mRNA after 5' processing.
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