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Originally published as Genetics Published Articles Ahead of Print on August 22, 2005.

Genetics, Vol. 171, 1305-1309, November 2005, Copyright © 2005
doi:10.1534/genetics.105.043661

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Mapping With a Few Plants: Using Selective Mapping for Microsatellite Saturation of the Prunus Reference Map

Werner Howad*, Toshiya Yamamoto{dagger}, Elisabeth Dirlewanger{ddagger}, Raffaele Testolin§, Patrick Cosson{ddagger}, Guido Cipriani§, Antonio J. Monforte*, Laura Georgi**, Albert G. Abbott** and Pere Arús*,1

* Departament de Genètica Vegetal, Laboratori de Genètica Molecular Vegetal, CSIC-IRTA, 08348 Cabrils (Barcelona), Spain, {dagger} National Institute of Fruit Tree Science, Tsukuba, Japan, {ddagger} INRA, Unité de Recherches sur les Espèces Fruitières et la Vigne, F-33 883 Villenave d'Ornon Cedex, France, § Dipartimento di Scienze Agrarie e Ambientali, University of Udine, 33100 Udine, Italy and ** Department of Genetics and Biochemistry, Clemson University, Clemson, South Carolina 29634

1 Corresponding author: Departament de Genètica Vegetal, Laboratori de Genètica Molecular Vegetal, CSIC-IRTA, Carretera de Cabrils s/n, 08348 Cabrils (Barcelona), Spain.
E-mail: pere.arus{at}irta.es

The concept of selective (or bin) mapping is used here for the first time, using as an example the Prunus reference map constructed with an almond x peach F2 population. On the basis of this map, a set of six plants that jointly defined 65 possible different genotypes for the codominant markers mapped on it was selected. Sixty-three of these joint genotypes corresponded to a single chromosomal region (a bin) of the Prunus genome, and the two remaining corresponded to two bins each. The 67 bins defined by these six plants had a 7.8-cM average length and a maximum individual length of 24.7 cM. Using a unit of analysis composed of these six plants, their F1 hybrid parent, and one of the parents of the hybrid, we mapped 264 microsatellite (or simple-sequence repeat, SSR) markers from 401 different microsatellite primer pairs. Bin mapping proved to be a fast and economic strategy that could be used for further map saturation, the addition of valuable markers (such as those based on microsatellites or ESTs), and giving a wider scope to, and a more efficient use of, reference mapping populations.




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