Originally published as Genetics Published Articles Ahead of Print on July 14, 2005.

Genetics, Vol. 171, 683-694, October 2005, Copyright © 2005
doi:10.1534/genetics.104.038885

Epistatic Pleiotropy and the Genetic Architecture of Covariation Within Early and Late-Developing Skull Trait Complexes in Mice

Jason B. Wolf^*,1, Larry J. Leamy, Eric J. Routman and James M. Cheverud

^* Faculty of Life Sciences, University of Manchester, Manchester M13 9PT, United Kingdom, Department of Biology, University of North Carolina, Charlotte, North Carolina 28223, Department of Biology, San Francisco State University, San Francisco, California 94132 and Department of Anatomy and Neurobiology, Washington University School of Medicine, St. Louis, Missouri 63110

¹ Corresponding author: Faculty of Life Sciences, 3.614 Stopford Bldg., University of Manchester, Oxford Rd., Manchester M13 9PT, United Kingdom.
E-mail: jason{at}evolutionarygenetics.org

The role of epistasis as a source of trait variation is well established, but its role as a source of covariation among traits (i.e., as a source of "epistatic pleiotropy") is rarely considered. In this study we examine the relative importance of epistatic pleiotropy in producing covariation within early and late-developing skull trait complexes in a population of mice derived from an intercross of the Large and Small inbred strains. Significant epistasis was found for several pairwise combinations of the 21 quantitative trait loci (QTL) affecting early developing traits and among the 20 QTL affecting late-developing traits. The majority of the epistatic effects were restricted to single traits but epistatic pleiotropy still contributed significantly to covariances. Because of their proportionally larger effects on variances than on covariances, epistatic effects tended to reduce within-group correlations of traits and reduce their overall degree of integration. The expected contributions of single-locus and two-locus epistatic pleiotropic QTL effects to the genetic covariance between traits were analyzed using a two-locus population genetic model. The model demonstrates that, for single-locus or epistatic pleiotropy to contribute to trait covariances in the study population, both traits must show the same pattern of single-locus or epistatic effects. As a result, a large number of the cases where loci show pleiotropic effects do not contribute to the covariance between traits in this population because the loci show a different pattern of effect on the different traits. In general, covariance patterns produced by single-locus and epistatic pleiotropy predicted by the model agreed well with actual values calculated from the QTL analysis. Nearly all single-locus and epistatic pleiotropic effects contributed positive components to covariances between traits, suggesting that genetic integration in the skull is achieved by a complex combination of pleiotropic effects.

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