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Originally published as Genetics Published Articles Ahead of Print on June 8, 2005.
Genetics, Vol. 171, 71-80, September 2005, Copyright © 2005
doi:10.1534/genetics.105.040584
The Heterotrimeric G-Protein GanB(
)-SfaD(ß)-GpgA(
) Is a Carbon Source Sensor Involved in Early cAMP-Dependent Germination in Aspergillus nidulans
Anne Lafon*,
Jeong-Ah Seo
,
Kap-Hoon Han,
Jae-Hyuk Yu and
Christophe d'Enfert*,1
* Unité Postulante Biologie et Pathogénicité Fongiques, INRA USC2019, Institut Pasteur, 75724 Paris Cedex 15, France and Department of Food Microbiology and Toxicology and Food Research Institute, Molecular and Environmental Toxicology Center, University of Wisconsin, Madison, Wisconsin 53706
1 Corresponding author: Unité Postulante Biologie et Pathogénicité Fongiques, INRA USC2019, Département Dynamique et Structure des Génomes, Institut Pasteur, 25, rue du Docteur Roux, 75724 Paris Cedex 15, France.
E-mail: denfert{at}pasteur.fr
The role of heterotrimeric G-proteins in cAMP-dependent germination of conidia was investigated in the filamentous ascomycete Aspergillus nidulans. We demonstrate that the G
-subunit GanB mediates a rapid and transient activation of cAMP synthesis in response to glucose during the early period of germination. Moreover, deletion of individual G-protein subunits resulted in defective trehalose mobilization and altered germination kinetics, indicating that GanB()-SfaD(ß)-GpgA(
) constitutes a functional heterotrimer and controls cAMP/PKA signaling in response to glucose as well as conidial germination. Further genetic analyses suggest that GanB plays a primary role in cAMP/PKA signaling, whereas the SfaD-GpgA (Gß) heterodimer is crucial for proper activation of GanB signaling sensitized by glucose. In addition, the RGS protein RgsA is also involved in regulation of the cAMP/PKA pathway and germination via attenuation of GanB signaling. Genetic epistatic analyses led us to conclude that all controls exerted by GanB()-SfaD(ß)-GpgA() on conidial germination are mediated through the cAMP/PKA pathway. Furthermore, GanB may function in sensing various carbon sources and subsequent activation of downstream signaling for germination.
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