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Originally published as Genetics Published Articles Ahead of Print on June 3, 2005.

Genetics, Vol. 171, 7-21, September 2005, Copyright © 2005
doi:10.1534/genetics.105.042598

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The Fission Yeast Schizosaccharomyces pombe Has Two Importin-{alpha} Proteins, Imp1p and Cut15p, Which Have Common and Unique Functions in Nucleocytoplasmic Transport and Cell Cycle Progression

Makoto Umeda*, Shahed Izaddoost*,1, Ian Cushman{dagger},{ddagger},2, Mary Shannon Moore{dagger},{ddagger},3 and Shelley Sazer*,{dagger},{ddagger},4

* Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, Texas 77030, {dagger} Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, Texas 77030 and {ddagger} The Graduate Program in Cell and Molecular Biology, Baylor College of Medicine, Houston, Texas 77030

4 Corresponding author: Department of Biochemistry and Molecular Biology, Baylor College of Medicine, 1 Baylor Plaza, Houston, TX 77030.
E-mail: ssazer{at}bcm.tmc.edu

The nuclear import of classical nuclear localization signal-containing proteins depends on importin-{alpha} transport receptors. In budding yeast there is a single importin-{alpha} gene and in higher eukaryotes there are multiple importin-{alpha}-like genes, but in fission yeast there are two: the previously characterized cut15 and the more recently identified imp1. Like other importin-{alpha} family members, Imp1p supports nuclear protein import in vitro. In contrast to cut15, imp1 is not essential for viability, but imp1{Delta} mutant cells exhibit a telophase delay and mild temperature-sensitive lethality. Differences in the cellular functions that depend on Imp1p and Cut15p indicate that they each have unique physiological roles. They also have common roles because the imp1{Delta} and the cut15-85 temperature-sensitive mutations are synthetically lethal; overexpression of cut15 partially suppresses the temperature sensitivity, but not the mitotic delay in imp1{Delta} cells; and overexpression of imp1 partially suppresses the mitotic defect in cut15-85 cells but not the loss of viability. Both Imp1p and Cut15p are required for the efficient nuclear import of both an SV40 nuclear localization signal-containing reporter protein and the Pap1p component of the stress response MAP kinase pathway. Imp1p and Cut15p are essential for efficient nuclear protein import in S. pombe.




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