- THIS ARTICLE
- Full Text
- Full Text (PDF)
-
All Versions of this Article:
genetics.104.038448v1
171/1/359 most recent - Alert me when this article is cited
- Alert me if a correction is posted
- SERVICES
- Email this article to a friend
- Similar articles in this journal
- Similar articles in PubMed
- Alert me to new issues of the journal
- Download to citation manager
- Reprints & Permissions
- CITING ARTICLES
- Citing Articles via HighWire
- Citing Articles via Google Scholar
- GOOGLE SCHOLAR
- Articles by Piepho, H.-P.
- Search for Related Content
- PUBMED
- PubMed Citation
- Articles by Piepho, H.-P.
Originally published as Genetics Published Articles Ahead of Print on June 14, 2005.
Genetics, Vol. 171, 359-364, September 2005, Copyright © 2005
doi:10.1534/genetics.104.038448
Optimal Allocation in Designs for Assessing Heterosis From cDNA Gene Expression Data
Hans-Peter Piepho1
Institut für Pflanzenbau und Grünland, Universität Hohenheim, 70599 Stuttgart, Germany
1 Address for correspondence: Institut für Pflanzenbau und Grünland, Universität Hohenheim, Fruwirthstrasse 23, 70599 Stuttgart, Germany.
E-mail: piepho{at}uni-hohenheim.de
Heterosis is defined as the superiority of a hybrid cross over its two parents. Plant and animals breeders have long been exploiting heterosis, but the causes of this phenomenon are as yet only partly understood. Recently, chip technology has opened up the opportunity to study heterosis at the gene expression level. This article considers the cDNA chip technology, which allows assaying two genotypes simultaneously on the same chip. Heterosis involves the response of at least three genotypes (two parents and their hybrid), so a chip or microarray constitutes an incomplete block, which raises a design problem specific to heterosis studies. The question to be answered is how genotype pairs should be allocated to chips. We address this design problem for two types of heterosis: midparent heterosis and better-parent heterosis. The general picture emerging from our results is that most of the resources should be allocated to parent-hybrid pairs, while chips with parent-parent pairs or hybrid-reciprocal pairs should be used sparingly or not at all.
This article has been cited by other articles:
 |
|
 |
|
  A. C. Lam, J. Fu, R. C. Jansen, C. S. Haley, and D.-J. de Koning Optimal Design of Genetic Studies of Gene Expression With Two-Color Microarrays in Outbred Crosses Genetics, November 1, 2008; 180(3): 1691 - 1698. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. J. M. Rosa, N. de Leon, and A. J. M. Rosa Review of microarray experimental design strategies for genetical genomics studies Physiol Genomics, December 13, 2006; 28(1): 15 - 23. [Abstract] [Full Text] [PDF]
|
|
|