Originally published as Genetics Published Articles Ahead of Print on June 21, 2005.

Genetics, Vol. 171, 211-225, September 2005, Copyright © 2005
doi:10.1534/genetics.105.044412

Quantitative Trait Loci Affecting the Difference in Pigmentation Between Drosophila yakuba and D. santomea

Mary Anna Carbone^*,1,2, Ana Llopart^,1,3, Matthew deAngelis^,4, Jerry A. Coyne and Trudy F. C. Mackay^*

^* Department of Genetics, North Carolina State University, Raleigh, North Carolina 27695 and Department of Ecology and Evolution, University of Chicago, Chicago, Illinois 60637

² Corresponding author: Department of Genetics, Room 3513, Gardner Hall, North Carolina State University, Raleigh, NC 27695.
E-mail: macarbon{at}unity.ncsu.edu

Using quantitative trait locus (QTL) mapping, we studied the genetic basis of the difference in pigmentation between two sister species of Drosophila: Drosophila yakuba, which, like other members of the D. melanogaster subgroup, shows heavy black pigmentation on the abdomen of males and females, and D. santomea, an endemic to the African island of São Tomé, which has virtually no pigmentation. Here we mapped four QTL with large effects on this interspecific difference in pigmentation: two on the X chromosome and one each on the second and third chromosomes. The same four QTL were detected in male hybrids in the backcrosses to both D. santomea and D. yakuba and in the female D. yakuba backcross hybrids. All four QTL exhibited strong epistatic interactions in male backcross hybrids, but only one pair of QTL interacted in females from the backcross to D. yabuka. All QTL from each species affected pigmentation in the same direction, consistent with adaptive evolution driven by directional natural selection. The regions delimited by the QTL included many positional candidate loci in the pigmentation pathway, including genes affecting catecholamine biosynthesis, melanization of the cuticle, and many additional pleiotropic effects.

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