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Originally published as Genetics Published Articles Ahead of Print on June 8, 2005.
Genetics, Vol. 170, 1737-1745, August 2005, Copyright © 2005
doi:10.1534/genetics.104.036178
Drosophila ERCC1 Is Required for a Subset of MEI-9-Dependent Meiotic Crossovers
Sarah J. Radford*,
Elizabeth Goley
,1,
Kimberly Baxter,2,
Susan McMahan and
Jeff Sekelsky*,,3
* Curriculum in Genetics and Molecular Biology, University of North Carolina, Chapel Hill, North Carolina 27599
Department of Biology, University of North Carolina, Chapel Hill, North Carolina 27599
3 Corresponding author: Department of Biology, CB 3280, 303 Fordham Hall, University of North Carolina, Chapel Hill, NC 27599-3280.
E-mail: sekelsky{at}unc.edu
Drosophila MEI-9 is the catalytic subunit of a DNA structure-specific endonuclease required for nucleotide excision repair (NER). The enzymatic activity of this endonuclease during NER requires the presence of a second, noncatalytic subunit called ERCC1. In addition to its role in NER, MEI-9 is required for the generation of most meiotic crossovers. To better understand the role of MEI-9 in crossover formation, we report here the characterization of the Drosophila Ercc1 gene. We created an Ercc1 mutant through homologous gene targeting. We find that Ercc1 mutants are identical to mei-9 mutants in sensitivity to DNA-damaging agents, but have a less severe reduction in the number of meiotic crossovers. MEI-9 protein levels are reduced in Ercc1 mutants; however, overexpression of MEI-9 is not sufficient to restore meiotic crossing over in Ercc1 mutants. We conclude that MEI-9 can generate some meiotic crossovers in an ERCC1-independent manner.
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