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Originally published as Genetics Published Articles Ahead of Print on June 8, 2005.
Genetics, Vol. 170, 1561-1574, August 2005, Copyright © 2005
doi:10.1534/genetics.105.041459
End3p-Mediated Endocytosis Is Required for Spore Wall Formation in Saccharomyces cerevisiae
Masayo Morishita and JoAnne Engebrecht1
Section of Molecular and Cellular Biology, University of California, Davis, California 95616
1 Corresponding author: Section of Molecular and Cellular Biology, 130 Briggs Hall, University of California, Davis, CA 95616.
E-mail: jengebrecht{at}ucdavis.edu
During sporulation in Saccharomyces cerevisiae, vesicles transported to the vicinity of spindle pole bodies are fused to each other to generate bilayered prospore membranes (PSMs). PSMs encapsulate the haploid nuclei that arise from the meiotic divisions and serve as platforms for spore wall deposition. Membrane trafficking plays an important role in supplying vesicles for these processes. The endocytosis-deficient mutant, end3
, sporulated poorly and the spores produced lost resistance to ether vapor, suggesting that END3-mediated endocytosis is important for sporulation. End3p-GFP localized to cell and spore peripheries in vegetative and sporulating cells and colocalized with actin structures. Correspondingly, the actin cytoskeleton appeared aberrant during sporulation in end3. Analysis of meiosis in end3 mutants revealed that the meiotic divisions occurred with wild-type kinetics. Furthermore, PSMs were assembled normally. However, the levels of proteins required for spore wall synthesis and components of the spore wall layers at spores were reduced, indicating that end3 mutants are defective in spore wall synthesis. Thus, END3-mediated endocytosis is important for spore wall formation. Additionally, cytological analyses suggest that trafficking between the plasma membrane and PSMs is important earlier during sporulation.
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