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Originally published as Genetics Published Articles Ahead of Print on May 23, 2005.
Genetics, Vol. 170, 1423-1426, July 2005, Copyright © 2005
doi:10.1534/genetics.105.042697
A New Reversion Assay for Measuring All Possible Base Pair Substitutions in Saccharomyces cerevisiae
Teresa-Marie Williams, Rebecca M. Fabbri, Jason W. Reeves and Gray F. Crouse1
Department of Biology, Emory University, Atlanta, Georgia 30322
1 Corresponding author: Department of Biology, Emory University, 1510 Clifton Rd., Atlanta, GA 30322-1100.
E-mail: gcrouse{at}biology.emory.edu
A TRP5-based reversion system that allows the rates of all possible base pair substitutions to be measured when the TRP5 locus is in both orientations relative to a defined origin of replication has been developed. This system should be useful for a wide variety of mutation and repair studies in yeast.