- THIS ARTICLE
- Full Text
- Full Text (PDF)
-
All Versions of this Article:
genetics.104.034587v1
170/3/1133 most recent - Alert me when this article is cited
- Alert me if a correction is posted
- SERVICES
- Email this article to a friend
- Similar articles in this journal
- Similar articles in PubMed
- Alert me to new issues of the journal
- Download to citation manager
- Reprints & Permissions
- CITING ARTICLES
- Citing Articles via HighWire
- Citing Articles via Google Scholar
- GOOGLE SCHOLAR
- Articles by Golovnin, A.
- Articles by Georgiev, P.
- Search for Related Content
- PUBMED
- PubMed Citation
- Articles by Golovnin, A.
- Articles by Georgiev, P.
Originally published as Genetics Published Articles Ahead of Print on November 1, 2004.
Genetics, Vol. 170, 1133-1142, July 2005, Copyright © 2005
doi:10.1534/genetics.104.034587
Drosophila Su(Hw) Insulator Can Stimulate Transcription of a Weakened yellow Promoter Over a Distance
Anton Golovnin*,
,
Elena Melnick*,
Alexander Mazur* and
Pavel Georgiev*,1
* Institute of Gene Biology, Russian Academy of Sciences, Moscow 119334, Russia
Center for Medical Studies of Oslo University, Moscow 199334, Russia
1 Corresponding author: Institute of Gene Biology, Russian Academy of Sciences, 34/5 Vavilov St., Moscow 119334, Russia.
E-mail: georgiev_p{at}mail.ru
The insulator element from the gypsy transposon is a DNA sequence that blocks activation of a promoter by a transcriptional enhancer when placed between them. The insulator contains reiterated binding sites for the Suppressor of Hairy-wing [Su(Hw)] zinc-finger protein. A protein encoded by another gene, modifier of mdg4 [mod(mdg4)], is also required for the enhancer-blocking activity of the Su(Hw) insulator. Here we present evidence that the Su(Hw) insulator activates a weakened yellow promoter at a distance. Deletion of the upstream promoter region (UPR), located close by the TATA box, significantly reduces yellow expression. The Su(Hw) insulator placed at different positions relative to the yellow promoter partially compensates for loss of the UPR. Su(Hw) is able to stimulate yellow expression even if it is located at a 5-kb distance from the promoter. The stimulatory activity depends on the number of Su(Hw)-binding sites. Mutational analysis demonstrates that only the DNA-binding domain and adjacent regions of the Su(Hw) protein are required for stimulation of yellow transcription.
This article has been cited by other articles:
 |
|
 |
|
  O. Kyrchanova, D. Chetverina, O. Maksimenko, A. Kullyev, and P. Georgiev Orientation-dependent interaction between Drosophila insulators is a property of this class of regulatory elements Nucleic Acids Res., December 1, 2008; 36(22): 7019 - 7028. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Pomerantseva, I. Biryukova, R. Silicheva, E. Savitskaya, A. Golovnin, and P. Georgiev Transposition of Regulatory Elements by P-Element-Mediated Rearrangements in Drosophila melanogaster Genetics, April 1, 2006; 172(4): 2283 - 2291. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Savitskaya, L. Melnikova, M. Kostuchenko, E. Kravchenko, E. Pomerantseva, T. Boikova, D. Chetverina, A. Parshikov, P. Zobacheva, E. Gracheva, et al. Study of Long-Distance Functional Interactions between Su(Hw) Insulators That Can Regulate Enhancer-Promoter Communication in Drosophila melanogaster Mol. Cell. Biol., February 1, 2006; 26(3): 754 - 761. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Kravchenko, E. Savitskaya, O. Kravchuk, A. Parshikov, P. Georgiev, and M. Savitsky Pairing between gypsy Insulators Facilitates the Enhancer Action in trans throughout the Drosophila Genome Mol. Cell. Biol., November 1, 2005; 25(21): 9283 - 9291. [Abstract] [Full Text] [PDF]
|
|