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Originally published as Genetics Published Articles Ahead of Print on May 23, 2005.
Genetics, Vol. 170, 1045-1062, July 2005, Copyright © 2005
doi:10.1534/genetics.104.040105
In Vivo Characterization of the Nonessential Budding Yeast Anaphase-Promoting Complex/Cyclosome Components Swm1p, Mnd2p and Apc9p
Andrew M. Page*,
,
Vicky Aneliunas,
John R. Lamb
,1 and
Philip Hieter,2
* Program in Biochemistry, Cellular, and Molecular Biology, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205
Department of Molecular Biology and Genetics, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205
Michael Smith Laboratories, University of British Columbia, Vancouver, British Columbia V6T 1Z4, Canada
2 Corresponding author: Michael Smith Laboratories, 2185 East Mall, Vancouver, BC V6T 124, Canada.
E-mail: hieter{at}msl.ubc.ca
We have examined the in vivo requirement of two recently identified nonessential components of the budding yeast anaphase-promoting complex, Swm1p and Mnd2p, as well as that of the previously identified subunit Apc9p. swm1
mutants exhibit synthetic lethality or conditional synthetic lethality with other APC/C subunits and regulators, whereas mnd2 mutants are less sensitive to perturbation of the APC/C. swm1 mutants, but not mnd2 mutants, exhibit defects in APC/C substrate turnover, both during the mitotic cell cycle and in 
-factor-arrested cells. In contrast, apc9 mutants exhibit only minor defects in substrate degradation in -factor-arrested cells. In cycling cells, degradation of Clb2p, but not Pds1p or Clb5p, is delayed in apc9. Our findings suggest that Swm1p is required for full catalytic activity of the APC/C, whereas the requirement of Mnd2p for APC/C function appears to be negligible under standard laboratory conditions. Furthermore, the role of Apc9p in APC/C-dependent ubiquitination may be limited to the proteolysis of a select number of substrates.
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