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Originally published as Genetics Published Articles Ahead of Print on March 2, 2005.

Genetics, Vol. 170, 95-106, May 2005, Copyright © 2005
doi:10.1534/genetics.104.036301

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Differential Activation of M26-Containing Meiotic Recombination Hot Spots in Schizosaccharomyces pombe

David W. Pryce*, Alexander Lorenz{dagger}, Julia B. Smirnova*,1, Josef Loidl{dagger} and Ramsay J. McFarlane*,2

* North West Cancer Research Fund Institute, University of Wales Bangor, Bangor LL57 2UW, United Kingdom
{dagger} Department of Chromosome Biology, University of Vienna, A-1030 Vienna, Austria

2 Corresponding author: North West Cancer Research Fund Institute, Memorial Bldg., University of Wales Bangor, Deiniol Rd., Bangor, Gwynedd LL57 2UW, United Kingdom.
E-mail: ramsay{at}sbs.bangor.ac.uk

Certain genomic loci, termed hot spots, are predisposed to undergo genetic recombination during meiosis at higher levels relative to the rest of the genome. The factors that specify hot-spot potential are not well understood. The M26 hot spot of Schizosaccharomyces pombe is dependent on certain trans activators and a specific nucleotide sequence, which can function as a hot spot in a position- and orientation-independent fashion within ade6. In this report we demonstrate that a linear element (LE) component, Rec10, has a function that is required for activation of some, but not all, M26-containing hot spots and from this we propose that, with respect to hot-spot activity, there are three classes of M26-containing sequences. We demonstrate that the localized sequence context in which the M26 heptamer is embedded is a major factor governing whether or not this Rec10 function is required for full hot-spot activation. Furthermore, we show that the rec10-144 mutant, which is defective in full activation of ade6-M26, but proficient for activation of other M26-containing hot spots, is also defective in the formation of LEs, suggesting an intimate link between higher-order chromatin structure and local influences on hot-spot activation.




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