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Originally published as Genetics Published Articles Ahead of Print on January 16, 2005.
Genetics, Vol. 169, 1391-1402, March 2005, Copyright © 2005
doi:10.1534/genetics.104.030817
SepBCTF4 Is Required for the Formation of DNA-Damage-Induced UvsCRAD51 Foci in Aspergillus nidulans
Scott E. Gygax*,1,
Camile P. Semighini
,
,
Gustavo H. Goldman and
Steven D. Harris,2
* Department of Microbiology, University of Connecticut Health Center, Farmington, Connecticut 06030-3205
Plant Science Initiative and Department of Plant Pathology, University of Nebraska, Lincoln, Nebraska 68588-0660
Departmento de Ciencias Farmaceuticas, Faculdade de Ciencias Farmaceuticas de Ribeirao Preto, Universidade de Sao Paulo, Ribeirao Preto, CEP 14040-903 Sao Paulo, Brazil
2 Corresponding author: Plant Science Initiative, University of Nebraska, N234 Beadle Center, Lincoln, NE 68588-0660.
E-mail: sharri1{at}unlnotes.unl.edu
SepB is an essential, conserved protein required for chromosomal DNA metabolism in Aspergillus nidulans. Homologs of SepB include yeast Ctf4p and human hAnd-1. Molecular and bioinformatic characterization of these proteins suggests that they act as molecular scaffolds. Furthermore, recent observations implicate the yeast family members in lagging-strand replication and the establishment of sister-chromatid cohesion. Here, we demonstrate that SepB functions in the A. nidulans DNA damage response. In particular, analysis of double mutants reveals that SepB is a member of the UvsCRAD51 epistasis group. In accord with this prediction, we show that UvsCRAD51 forms DNA-damage-induced nuclear foci in a manner that requires SepB function. We also provide evidence that implicates SepB in sister-chromatid cohesion, thereby suggesting that cohesion may play a role in regulating the localization and/or assembly of UvsCRAD51 complexes.
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