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Originally published as Genetics Published Articles Ahead of Print on January 16, 2005.
Genetics, Vol. 169, 1305-1310, March 2005, Copyright © 2005
doi:10.1534/genetics.104.033407
Trans Events Associated With Crossovers Are Revealed in the Absence of Mismatch Repair Genes in Saccharomyces cerevisiae
Eva R. Hoffmann and Rhona H. Borts1
Department of Genetics, University of Leicester, Leicester LE1 7RH, United Kingdom
1 Corresponding author: Department of Genetics, University Rd., Adrian Bldg., University of Leicester, Leicester LE1 7RH, United Kingdom.
E-mail: rhb7{at}le.ac.uk
Genetic analysis of recombination in Saccharomyces cerevisiae has revealed products with structures not predicted by the double-strand break repair model of meiotic recombination. A particular type of recombinant containing trans heteroduplex DNA has been observed at two loci. Trans events were originally identified only in tetrads in which the non-Mendelian segregations were not associated with a crossover. Because of this, these events were proposed to have arisen from the unwinding of double Holliday junctions. Previous studies used palindromes, refractory to mismatch repair, as genetic markers whereas we have used a complementary approach of deleting mismatch repair proteins to identify heteroduplex DNA. We found that the markers occurred in trans and were associated with crossovers. In both mlh1
and msh2 strains, the frequency of trans events associated with a crossover exceeded that predicted from the random association of crossovers with noncrossover trans events. We propose two different models to account for trans events associated with crossovers and discuss the relevance to wild-type DSB repair.
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