- THIS ARTICLE
- Full Text
- Full Text (PDF)
-
All Versions of this Article:
genetics.104.034603v1
169/3/1215 most recent - Alert me when this article is cited
- Alert me if a correction is posted
- SERVICES
- Similar articles in this journal
- Similar articles in PubMed
- Alert me to new issues of the journal
- Download to citation manager
- Reprints & Permissions
- CITING ARTICLES
- Citing Articles via HighWire
- Citing Articles via Google Scholar
- GOOGLE SCHOLAR
- Articles by Bose, S.
- Articles by Zitomer, R. S.
- Search for Related Content
- PUBMED
- PubMed Citation
- Articles by Bose, S.
- Articles by Zitomer, R. S.
Originally published as Genetics Published Articles Ahead of Print on November 15, 2004.
Genetics, Vol. 169, 1215-1226, March 2005, Copyright © 2005
doi:10.1534/genetics.104.034603
Genetic Factors That Regulate the Attenuation of the General Stress Response of Yeast
Sohini Bose, James A. Dutko and Richard S. Zitomer1
Department of Biological Sciences, University at Albany/SUNY, Albany, New York 12222
1 Corresponding author: Department of Biological Sciences, University at Albany/SUNY, 1400 Washington Ave., Albany, NY 12222.
E-mail: rz144{at}albany.edu
The general stress response of yeast involves the induction of 
200 genes in response to any one of several stresses. These genes are activated by Msn2 and repressed by the Srb10 kinase, a member of the mediator complex. Normally, Msn2 is exported from the nucleus, and Srb10 represses STRE gene expression. Under stress, Msn2 relocalizes to the nucleus and, with the relief of Srb10 repression, activates transcription. The stress response is rapid, but quickly attenuated. We show here that this attenuation is due to a nuclear-dependent degradation of Msn2. Msn2 rapidly disappeared from cells after heat or osmotic shock. This disappearance was not due to a change in MSN2 RNA levels, which remain constant during stress. Pulse-chase experiments confirmed the stress-dependent Msn2 degradation. The levels of Msn2 were significantly reduced in msn5 deletion cells that have been shown to constitutively retain Msn2 in the nucleus. The degradation was Srb10-dependent; Msn2 was not degraded in an srb10 deletion mutant. An Msn2 internal deletion mutant was insensitive to Srb10 repression, but was degraded by the Srb10-dependent mechanism. Thus, this mutation uncoupled Srb10 repression from degradation.
This article has been cited by other articles:
 |
|
 |
|
  T. Y. Erkina, P. A. Tschetter, and A. M. Erkine Different Requirements of the SWI/SNF Complex for Robust Nucleosome Displacement at Promoters of Heat Shock Factor and Msn2- and Msn4-Regulated Heat Shock Genes Mol. Cell. Biol., February 15, 2008; 28(4): 1207 - 1217. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Lenssen, N. Azzouz, A. Michel, E. Landrieux, and M. A. Collart The Ccr4-Not Complex Regulates Skn7 through Srb10 Kinase Eukaryot. Cell, December 1, 2007; 6(12): 2251 - 2259. [Abstract] [Full Text] [PDF]
|
|