Originally published as Genetics Published Articles Ahead of Print on November 1, 2004.

Genetics, Vol. 169, 967-979, February 2005, Copyright © 2005
doi:10.1534/genetics.104.033209

Detection of Chromosomal Rearrangements Derived From Homeologous Recombination in Four Mapping Populations of Brassica napus L.

Joshua A. Udall^1,2, Pablo A. Quijada¹ and Thomas C. Osborn³

Department of Agronomy, University of Wisconsin, Madison, Wisconsin 53706

³ Corresponding author: Department of Agronomy, University of Wisconsin,1575 Linden Dr., Madison, WI 53706.
E-mail: tcosborn{at}wisc.edu

Genetic maps of Brassica napus were constructed from four segregating populations of doubled haploid lines. Each mapping population had the same male parent and used the same set of RFLP probes, facilitating the construction of a consensus map. Chromosomal rearrangements were identified in each population by molecular marker analysis and were classified as de novo homeologous nonreciprocal transpositions (HNRTs), preexisting HNRTs, and homeologous reciprocal transpositions (HRTs). Ninety-nine de novo HNRTs were identified by the presence of a few lines having duplication of a chromosomal region and loss of the corresponding homeologous region. These de novo HNRTs were more prevalent in one population that had a resynthesized B. napus as a parent. Preexisting HNRTs were identified by fragment duplication or fragment loss in many DH lines due to the segregation of HNRTs preexisting in one of the parents. Nine preexisting HNRTs were identified in the three populations involving natural B. napus parents, which likely originated from previous homeologous exchanges. The male parent had a previously described HRT between N7 and N16, which segregated in each population. These data suggest that chromosomal rearrangements caused by homeologous recombination are widespread in B. napus. The effects of these rearrangements on allelic and phenotypic diversity are discussed.

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