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Originally published as Genetics Published Articles Ahead of Print on November 15, 2004.

Genetics, Vol. 169, 607-617, February 2005, Copyright © 2005
doi:10.1534/genetics.104.035964

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RNA Silencing in Aspergillus nidulans Is Independent of RNA-Dependent RNA Polymerases

T. M. Hammond and N. P. Keller1

Department of Plant Pathology, University of Wisconsin, Madison, Wisconsin 53706

1 Corresponding author: Department of Plant Pathology, University of Wisconsin, 1630 Linden Dr., Madison, WI 53706.
E-mail: npk{at}plantpath.wisc.edu

The versatility of RNA-dependent RNA polymerases (RDRPs) in eukaryotic gene silencing is perhaps best illustrated in the kingdom Fungi. Biochemical and genetic studies of Schizosaccharomyces pombe and Neurospora crassa show that these types of enzymes are involved in a number of fundamental gene-silencing processes, including heterochromatin regulation and RNA silencing in S. pombe and meiotic silencing and RNA silencing in N. crassa. Here we show that Aspergillus nidulans, another model fungus, does not require an RDRP for inverted repeat transgene (IRT)-induced RNA silencing. However, RDRP requirements may vary within the Aspergillus genus as genomic analysis indicates that A. nidulans, but not A. fumigatus or A. oryzae, has lost a QDE-1 ortholog, an RDRP associated with RNA silencing in N. crassa. We also provide evidence suggesting that 5' -> 3' transitive RNA silencing is not a significant aspect of A. nidulans IRT-RNA silencing. These results indicate a lack of conserved kingdom-wide requirements for RDRPs in fungal RNA silencing.




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